Identification of the topology and functional domains of PAQR10
文献类型：期刊论文
| 作者 | Jin, Ting; Xu, Daqian; Ding, QiuRong（丁秋蓉） ; Zhang, Yixuan; Mao, Chenqian; Pan, Yi（潘怡）; Wang, ZhenZhen（王甄真）; Chen, Yan（陈雁）
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| 刊名 | BIOCHEMICAL JOURNAL
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| 出版日期 | 2012 |
| 卷号 | 443期号:0页码:643-653 |
| 关键词 | compartmentalization extracellular-signal-regulated kinase (ERK) Golgi apparatus progestin and adipoQ receptor 10 (PAQR 10) Ras |
| 英文摘要 | PAQR10 (progestin and adipoQ receptor 10) is a Golgi-localized protein that is able to enhance the retention and activation of Ras proteins in the Golgi apparatus, subsequently leading to a sustained ERK (extracellular-signal-regulated kinase) signalling. However, little is known about the topology and functional domains of PAQR10. In the present study, we extensively dissected and analysed the structure of PAQR10. The topology analysis reveals that PAQR10 is an integral membrane protein with its N-terminus facing the cytosol. Multiple domains, including the membrane-proximal region at the N-terminus, the membrane-proximal region at the C-terminus and the three loops facing the cytosol, were found to be required for PAQR10 to reside in the Golgi apparatus, to stimulate ERK phosphorylation and to tether Ras to the Golgi apparatus. Furthermore, when PAQR10 was artificially forced to be expressed in the endoplasmic reticulum, it could neither mobilize Ras to the Golgi apparatus nor increase ERK phosphorylation. Finally, the PAQR10 mutants that lost Golgi localization failed to promote differentiation of PC12 cells. Collectively, the results of the present study indicate that Golgi localization is indispensable for PAQR10 to implement its regulatory functions in the Ras signalling cascade. |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | MEMBRANE PROGESTIN RECEPTOR ; TRANS-GOLGI NETWORK ; ENDOPLASMIC-RETICULUM ; ADIPONECTIN RECEPTORS ; PROTEIN ; RKTG ; LOCALIZATION ; APPARATUS ; CLONING ; CALNEXIN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000303944200006 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/70]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所_信号转导与营养相关疾病研究组 |
| 推荐引用方式 GB/T 7714 | Jin, Ting,Xu, Daqian,Ding, QiuRong,et al. Identification of the topology and functional domains of PAQR10[J]. BIOCHEMICAL JOURNAL,2012,443(0):643-653. |
| APA | Jin, Ting.,Xu, Daqian.,Ding, QiuRong.,Zhang, Yixuan.,Mao, Chenqian.,...&Chen, Yan.(2012).Identification of the topology and functional domains of PAQR10.BIOCHEMICAL JOURNAL,443(0),643-653. |
| MLA | Jin, Ting,et al."Identification of the topology and functional domains of PAQR10".BIOCHEMICAL JOURNAL 443.0(2012):643-653. |
入库方式： OAI收割
来源：上海营养与健康研究所
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