PAQR10 and PAQR11 mediate Ras signaling in the Golgi apparatus
文献类型：期刊论文
| 作者 | Jin, Ting; Ding, QiuRong（丁秋蓉） ; Huang, Heng; Xu, Daqian; Jiang, Yuhui; Zhou, Ben; Li, Zhenghu; Jiang, Xiaomeng; He, Jing; Liu, Weizhong
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| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2012 |
| 卷号 | 22期号:4页码:661-676 |
| 关键词 | Ras Signal transduction Golgi apparatus compartmentalization ERK |
| 英文摘要 | Ras plays a pivotal role in many cellular activities, and its subcellular compartmentalization provides spatial and temporal selectivity. Here we report a mode of spatial regulation of Ras signaling in the Golgi apparatus by two highly homologous proteins PAQR10 and PAQR11 of the progestin and AdipoQ receptors family. PAQR10 and PAQR11 are exclusively localized in the Golgi apparatus. Overexpression of PAQR10/PAQR11 stimulates basal and EGF-induced ERK phosphorylation and increases the expression of ERK target genes in a dose-dependent manner. Overexpression of PAQR10/PAQR11 markedly elevates Golgi localization of HRas, NRas and KRas4A, but not KRas4B. PAQR10 and PAQR11 can also interact with HRas, NRas and KRas4A, but not KRas4B. The increased Ras protein at the Golgi apparatus by overexpression of PAQR10/PAQR11 is in an active state. Consistently, knockdown of PAQR10 and PAQR11 reduces EGF-stimulated ERK phosphorylation and Ras activation at the Golgi apparatus. Intriguingly, PAQR10 and PAQR11 are able to interact with RasGRP1, a guanine nucleotide exchange protein of Ras, and increase Golgi localization of RasGRP1. The C1 domain of RasGRP1 is both necessary and sufficient for the interaction of RasGRP1 with PAQR10/PAQR11. The simulation of ERK phosphorylation by overexpressed PAQR10/PAQR11 is abrogated by downregulation of RasGRP1. Furthermore, differentiation of PC12 cells is significantly enhanced by overexpression of PAQR10/PAQR11. Collectively, this study uncovers a new paradigm of spatial regulation of Ras signaling in the Golgi apparatus by PAQR10 and PAQR11. |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | PROTEIN-KINASE-C ; ACTIVATED RECEPTOR-GAMMA ; PLASMA-MEMBRANE ; ENDOPLASMIC-RETICULUM ; ADIPONECTIN RECEPTORS ; H-RAS ; DIFFERENTIALLY TARGETS ; EXCHANGE FACTORS ; CAAX MOTIF ; K-RAS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000302351800008 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/71]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所_信号转导与营养相关疾病研究组 |
| 推荐引用方式 GB/T 7714 | Jin, Ting,Ding, QiuRong,Huang, Heng,et al. PAQR10 and PAQR11 mediate Ras signaling in the Golgi apparatus[J]. CELL RESEARCH,2012,22(4):661-676. |
| APA | Jin, Ting.,Ding, QiuRong.,Huang, Heng.,Xu, Daqian.,Jiang, Yuhui.,...&Chen, Yan.(2012).PAQR10 and PAQR11 mediate Ras signaling in the Golgi apparatus.CELL RESEARCH,22(4),661-676. |
| MLA | Jin, Ting,et al."PAQR10 and PAQR11 mediate Ras signaling in the Golgi apparatus".CELL RESEARCH 22.4(2012):661-676. |
入库方式： OAI收割
来源：上海营养与健康研究所
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