Regulation of G-Protein Signaling by RKTG via Sequestration of the G beta gamma Subunit to the Golgi Apparatus
文献类型：期刊论文
| 作者 | Jiang, Yuhui; Xie, Xiaoduo; Zhang, Yixuan; Luo, Xiaolin; Wang, Xiao; Fan, Fengjuan; Zheng, Dawei; Wang, ZhenZhen（王甄真）; Chen, Yan（陈雁）
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| 刊名 | MOLECULAR AND CELLULAR BIOLOGY
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| 出版日期 | 2010 |
| 卷号 | 30期号:1页码:78-90 |
| 英文摘要 | Upon ligand binding, G-protein-coupled receptors (GPCRs) impart the signal to heterotrimeric G proteins composed of alpha, beta, and gamma subunits, leading to dissociation of the G alpha subunit from the G beta gamma subunit. While the G alpha subunit is imperative for downstream signaling, the G beta gamma subunit, in its own right, mediates a variety of cellular responses such as GPCR desensitization via recruiting GRK to the plasma membrane and AKT stimulation. Here we report a mode of spatial regulation of the G beta gamma subunit through alteration in subcellular compartmentation. RKTG (Raf kinase trapping to Golgi apparatus) is a newly characterized membrane protein specifically localized at the Golgi apparatus. The N terminus of RKTG interacts with G beta and tethers G beta gamma to the Golgi apparatus. Overexpression of RKTG impedes the interaction of G beta gamma with GRK2, abrogates the ligand-induced change of subcellular distribution of GRK2, reduces isoproterenol-stimulated phosphorylation of the beta 2-adrenergic receptor (beta 2AR), and alters beta 2AR desensitization. In addition, RKTG inhibits G beta gamma- and ligand-mediated AKT phosphorylation that is enhanced in cells with downregulation of RKTG. Silencing of RKTG also alters GRK2 internalization and compromises ligand-induced G beta translocation to the Golgi apparatus. Taken together, our results reveal that RKTG can modulate GPCR signaling through sequestering G beta gamma to the Golgi apparatus and thereby attenuating the functions of G beta gamma. |
| 类目[WOS] | Biochemistry & Molecular Biology ; Cell Biology |
| 关键词[WOS] | ADRENERGIC-RECEPTOR KINASE ; HETEROTRIMERIC G-PROTEINS ; PLASMA-MEMBRANE ; COMPLEX TRANSLOCATION ; COUPLED RECEPTORS ; ACTIVATION ; CELLS ; TRAFFICKING ; P110-GAMMA ; ISOFORM |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000272569200006 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/84]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所_信号转导与营养相关疾病研究组 |
| 推荐引用方式 GB/T 7714 | Jiang, Yuhui,Xie, Xiaoduo,Zhang, Yixuan,et al. Regulation of G-Protein Signaling by RKTG via Sequestration of the G beta gamma Subunit to the Golgi Apparatus[J]. MOLECULAR AND CELLULAR BIOLOGY,2010,30(1):78-90. |
| APA | Jiang, Yuhui.,Xie, Xiaoduo.,Zhang, Yixuan.,Luo, Xiaolin.,Wang, Xiao.,...&Chen, Yan.(2010).Regulation of G-Protein Signaling by RKTG via Sequestration of the G beta gamma Subunit to the Golgi Apparatus.MOLECULAR AND CELLULAR BIOLOGY,30(1),78-90. |
| MLA | Jiang, Yuhui,et al."Regulation of G-Protein Signaling by RKTG via Sequestration of the G beta gamma Subunit to the Golgi Apparatus".MOLECULAR AND CELLULAR BIOLOGY 30.1(2010):78-90. |
入库方式： OAI收割
来源：上海营养与健康研究所
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