Endocytosis of adiponectin receptor 1 through a clathrin- and Rab5-dependent pathway
文献类型：期刊论文
| 作者 | Ding, QiuRong（丁秋蓉） ; Wang, ZhenZhen（王甄真）; Chen, Yan（陈雁）
|
| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2009 |
| 卷号 | 19期号:3页码:317-327 |
| 关键词 | adiponectin adiponectin receptors clathrin endocytosis Rab5 |
| 英文摘要 | In eukaryotic cells, receptor endocytosis is a key event regulating signaling transduction. Adiponectin receptors belong to a new receptor family that is distinct from G-protein-coupled receptors and has critical roles in the pathogenesis of diabetes and metabolic syndrome. Here, we analyzed the endocytosis of adiponectin and adiponectin receptor 1 (AdipoR1) and found that they are both internalized into transferrin-positive compartments that follow similar traffic routes. Blocking clathrin-mediated endocytosis by expressing Eps15 mutants or depleting K+ trapped AdipoR1 at the plasma membrane, and K+ depletion abolished adiponectin internalization, indicating that the endocytosis of AdipoR1 and adiponectin is clathrin-dependent. Depletion of K+ and overexpression of Eps15 mutants enhance adiponectin-stimulated AMP-activated protein kinase phosphorylation, suggesting that the endocytosis of AdipoR1 might downregulate adiponectin signaling. In addition, AdipoR1 colocalizes with the small GTPase Rab5, and a dominant negative Rab5 abrogates AdipoR1 endocytosis. These data indicate that AdipoR1 is internalized through a clathrin-and Rab5-dependent pathway and that endocytosis may play a role in the regulation of adiponectin signaling. |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | PROTEIN-COUPLED RECEPTORS ; EPIDERMAL-GROWTH-FACTOR ; TGF-BETA RECEPTOR ; COATED PIT FORMATION ; MEDIATED ENDOCYTOSIS ; EGF RECEPTOR ; SIGNAL-TRANSDUCTION ; RAB5 ; INTERNALIZATION ; APPL1 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000265700000007 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/88]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所_信号转导与营养相关疾病研究组 |
| 推荐引用方式 GB/T 7714 | Ding, QiuRong,Wang, ZhenZhen,Chen, Yan. Endocytosis of adiponectin receptor 1 through a clathrin- and Rab5-dependent pathway[J]. CELL RESEARCH,2009,19(3):317-327. |
| APA | Ding, QiuRong,Wang, ZhenZhen,&Chen, Yan.(2009).Endocytosis of adiponectin receptor 1 through a clathrin- and Rab5-dependent pathway.CELL RESEARCH,19(3),317-327. |
| MLA | Ding, QiuRong,et al."Endocytosis of adiponectin receptor 1 through a clathrin- and Rab5-dependent pathway".CELL RESEARCH 19.3(2009):317-327. |
入库方式： OAI收割
来源：上海营养与健康研究所
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