SnoVectors for nuclear expression of RNA
文献类型：期刊论文
| 作者 | Yin, QF; Hu, SB; Xu, YF; Yang, L; Carmichael, GG; Chen, LL |
| 刊名 | NUCLEIC ACIDS RESEARCH
 |
| 出版日期 | 2015 |
| 卷号 | 43期号:1页码:e5-e5 |
| 通讯作者 | Chen, LL (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,linglingchen@sibcb.ac.cn |
| 英文摘要 | Many long noncoding RNAs (lncRNAs) are constrained to the nucleus to exert their functions. However, commonly used vectors that were designed to express mRNAs have not been optimized for the study of nuclear RNAs. We reported recently that sno-lncRNAs are not capped or polyadenylated but rather are terminated on each end by snoRNAs and their associated proteins. These RNAs are processed from introns and are strictly confined to the nucleus. Here we have used these features to design expression vectors that can stably express virtually any sequence of interest and constrain its accumulation to the nucleus. Further, these RNAs appear to retain normal nuclear associations and function. SnoVectors should be useful in conditions where nuclear RNA function is studied or where export to the cytoplasm needs to be avoided. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | LONG NONCODING RNAS ; IDENTIFICATION ; DOMAINS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000350207100005 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/5]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Yin, QF,Hu, SB,Xu, YF,et al. SnoVectors for nuclear expression of RNA[J]. NUCLEIC ACIDS RESEARCH,2015,43(1):e5-e5. |
| APA | Yin, QF,Hu, SB,Xu, YF,Yang, L,Carmichael, GG,&Chen, LL.(2015).SnoVectors for nuclear expression of RNA.NUCLEIC ACIDS RESEARCH,43(1),e5-e5. |
| MLA | Yin, QF,et al."SnoVectors for nuclear expression of RNA".NUCLEIC ACIDS RESEARCH 43.1(2015):e5-e5. |
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