RAG1-mediated ubiquitylation of histone H3 is required for chromosomal V(D)J recombination
文献类型：期刊论文
| 作者 | Deng, ZM; Liu, HF; Liu, XL |
| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2015 |
| 卷号 | 25期号:2页码:181-192 |
| 关键词 | RAG1 ubiquitylation V(D)J recombination histone H3 |
| 通讯作者 | Liu, XL (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Cell Biol, Shanghai 200031, Peoples R China.,liux@sibs.ac.cn |
| 英文摘要 | RAG1 and RAG2 proteins are key components in V(D)J recombination. The core region of RAG1 is capable of catalyzing the recombination reaction; however, the biological function of non-core RAG1 remains largely unknown. Here, we show that in a murine-model carrying the RAG1 ring-finger conserved cysteine residue mutation (C325Y), V(D)J recombination was abrogated at the cleavage step, and this effect was accompanied by decreased mono-ubiquitylation of histone H3. Further analyses suggest that un-ubiquitylated histone H3 restrains RAG1 to the chromatin by interacting with the N-terminal 218 amino acids of RAG1. Our data provide evidence for a model in which ubiquitylation of histone H3 mediated by the ring-finger domain of RAG1 triggers the release of RAG1, thus allowing its transition into the cleavage phase. Collectively, our findings reveal that the non-core region of RAG1 facilitates chromosomal V(D)J recombination in a ubiquitylation-dependent pathway. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | N-TERMINAL DOMAIN ; UBIQUITIN LIGASE ; RAG1 ; DNA ; CLEAVAGE ; COMPLEX ; PROTEINS ; SEQUENCE ; SIGNAL ; GENES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000349334100007 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/22]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Deng, ZM,Liu, HF,Liu, XL. RAG1-mediated ubiquitylation of histone H3 is required for chromosomal V(D)J recombination[J]. CELL RESEARCH,2015,25(2):181-192. |
| APA | Deng, ZM,Liu, HF,&Liu, XL.(2015).RAG1-mediated ubiquitylation of histone H3 is required for chromosomal V(D)J recombination.CELL RESEARCH,25(2),181-192. |
| MLA | Deng, ZM,et al."RAG1-mediated ubiquitylation of histone H3 is required for chromosomal V(D)J recombination".CELL RESEARCH 25.2(2015):181-192. |
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