A Vero-cell-adapted vaccine donor strain of influenza A virus generated by serial passages
文献类型：期刊论文
| 作者 | Hu, WB; Zhang, H; Han, QL; Li, L; Chen, YX; Xia, NS; Chen, Z; Shu, YL; Xu, K; Sun, B |
| 刊名 | VACCINE
 |
| 出版日期 | 2015 |
| 卷号 | 33期号:2页码:374-381 |
| 关键词 | Influenza A virus vaccine Vero-cell adaptation Serial passages |
| 通讯作者 | Xu, K (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Pasteur Shanghai, Key Lab Mol Virol & Immunol, Shanghai 200031, Peoples R China.,kxu@sibs.ac.cn |
| 英文摘要 | A cell culture-based vaccine production system is preferred for the large-scale production of influenza vaccines and has advantages for generating vaccines against highly pathogenic influenza A viruses. Vero cells have been widely used in human vaccine manufacturing, and the safety of these cells has been well demonstrated. However, the most commonly used influenza-vaccine donor virus, A/Puerto Rico/8/1934 (PR8) virus, does not grow efficiently in Vero cells. Therefore, we adapted the PR8 virus to Vero cells by continuous passaging, and a high-growth strain was obtained after 20 passages. Sequence analysis and virological assays of the adapted strain revealed that mutations in four viral internal genes (NP, PB1, PA and NS1) were sufficient for adaptation. The recombinant virus harboring these mutations (PR8-4mut) displayed accelerated viral transport into the nucleus and increased RNP activity. Importantly, the PR8-4mut could serve as a backbone donor virus to support the growth of the H7N1, H9N2 and H5N1 avian viruses and the H1N1 and H3N2 human viruses in Vero cells without changing its pathogenicity in either chicken embryos or mice. Thus, our work describes the generation of a Vero-adapted, high-yield PR8-4mut virus that may serve as a promising candidate for an influenza-vaccine donor virus. (C) 2014 Elsevier Ltd. All rights reserved. |
| 学科主题 | Immunology ; Research & Experimental Medicine |
| 类目[WOS] | Immunology ; Medicine, Research & Experimental |
| 关键词[WOS] | HIGH-GROWTH ; ENHANCED GROWTH ; SEED VIRUSES ; B VIRUS ; REPLICATION ; RNA ; REASSORTMENT ; PLATFORM ; NUCLEUS ; CULTURE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000348268500016 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/25]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Hu, WB,Zhang, H,Han, QL,et al. A Vero-cell-adapted vaccine donor strain of influenza A virus generated by serial passages[J]. VACCINE,2015,33(2):374-381. |
| APA | Hu, WB.,Zhang, H.,Han, QL.,Li, L.,Chen, YX.,...&Sun, B.(2015).A Vero-cell-adapted vaccine donor strain of influenza A virus generated by serial passages.VACCINE,33(2),374-381. |
| MLA | Hu, WB,et al."A Vero-cell-adapted vaccine donor strain of influenza A virus generated by serial passages".VACCINE 33.2(2015):374-381. |
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