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m(6)A RNA Methylation Is Regulated by MicroRNAs and Promotes Reprogramming to Pluripotency
文献类型：期刊论文
| 作者 | Chen, T; Hao, YJ; Zhang, Y; Li, MM; Wang, M; Han, WF; Wu, YS; Lv, Y; Hao, J; Wang, LB |
| 刊名 | CELL STEM CELL
 |
| 出版日期 | 2015 |
| 卷号 | 16期号:3页码:289-301 |
| 通讯作者 | Wang, XJ (reprint author), Chinese Acad Sci, Inst Genet & Dev Biol, Collaborat Innovat Ctr Genet & Dev, Key Lab Genet Network Biol, Beijing 100101, Peoples R China.,xjwang@genetics.ac.cn |
| 英文摘要 | N-6-methyladenosine (m(6)A) has been recently identified as a conserved epitranscriptomic modification of eukaryotic mRNAs, but its features, regulatory mechanisms, and functions in cell reprogramming are largely unknown. Here, we report m(6)A modification profiles in the mRNA transcriptomes of four cell types with different degrees of pluripotency. Comparative analysis reveals several features of m(6)A, especially gene-and cell-type-specific m(6)A mRNA modifications. We also show that microRNAs (miRNAs) regulate m(6)A modification via a sequence pairing mechanism. Manipulation of miRNA expression or sequences alters m(6)A modification levels through modulating the binding of METTL3 methyltransferase to mRNAs containing miRNA targeting sites. Increased m(6)A abundance promotes the reprogramming of mouse embryonic fibroblasts (MEFs) to pluripotent stem cells; conversely, reduced m(6)A levels impede reprogramming. Our results therefore uncover a role for miRNAs in regulating m(6)A formation of mRNAs and provide a foundation for future functional studies of m(6)A modification in cell reprogramming. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell & Tissue Engineering ; Cell Biology |
| 关键词[WOS] | EMBRYONIC STEM-CELLS ; ARABIDOPSIS MESSENGER-RNA ; ARGONAUTE PROTEINS ; NUCLEAR-RNA ; YTH DOMAIN ; REVEALS ; N6-METHYLADENOSINE ; N-6-METHYLADENOSINE ; SITES ; M6A |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000350747700011 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/33]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Chen, T,Hao, YJ,Zhang, Y,et al. m(6)A RNA Methylation Is Regulated by MicroRNAs and Promotes Reprogramming to Pluripotency[J]. CELL STEM CELL,2015,16(3):289-301. |
| APA | Chen, T.,Hao, YJ.,Zhang, Y.,Li, MM.,Wang, M.,...&Zhou, Q.(2015).m(6)A RNA Methylation Is Regulated by MicroRNAs and Promotes Reprogramming to Pluripotency.CELL STEM CELL,16(3),289-301. |
| MLA | Chen, T,et al."m(6)A RNA Methylation Is Regulated by MicroRNAs and Promotes Reprogramming to Pluripotency".CELL STEM CELL 16.3(2015):289-301. |
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