The N-terminal leucine-zipper motif in PTRF/cavin-1 is essential and sufficient for its caveolae-association
文献类型：期刊论文
| 作者 | Wei, Z; Zou, XL; Wang, HZ; Tei, JG; Wu, Y; Liao, I |
| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| 出版日期 | 2015 |
| 卷号 | 456期号:3页码:750-756 |
| 关键词 | PTRF/cavin-1 Caveolae Nuclear localization Phosphorylation Cell migration |
| 通讯作者 | Liao, I (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Cell Biol Lab, Shanghai 200031, Peoples R China.,kliao@sibs.ac.cn |
| 英文摘要 | PTRF/cavin-1 is a protein of two lives. Its reported functions in ribosomal RNA synthesis and in caveolae formation happen in two different cellular locations: nucleus vs. plasma membrane. Here, we identified that the N-terminal leucine-zipper motif in PTRF/cavin-1 was essential for the protein to be associated with caveolae in plasma membrane. It could counteract the effect of nuclear localization sequence in the molecule (AA 235-251). Deletion of this leucine-zipper motif from PTRF/cavin-1 caused the mutant to be exclusively localized in nuclei. The fusion of this leucine-zipper motif with histone 2A, which is a nuclear protein, could induce the fusion protein to be exported from nucleus. Cell migration was greatly inhibited in PTRF/cavin-1(-/-) mouse embryonic fibroblasts (MEFs). The inhibited cell motility could only be rescued by exogenous cavin-1 but not the leucine-zipper motif deleted cavin-1 mutant. Plasma membrane dynamics is an important factor in cell motility control. Our results suggested that the membrane dynamics in cell migration is affected by caveolae associated PTRF/cavin-1. (C) 2014 Elsevier Inc. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology ; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | TRANSCRIPT-RELEASE FACTOR ; POLYMERASE-I ; GENERALIZED LIPODYSTROPHY ; PTRF MUTATIONS ; PROTEIN ; CAVIN ; COMPLEXES ; ADIPOCYTES ; PROTEOMICS ; INTERACTS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000348973300008 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/42]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wei, Z,Zou, XL,Wang, HZ,et al. The N-terminal leucine-zipper motif in PTRF/cavin-1 is essential and sufficient for its caveolae-association[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2015,456(3):750-756. |
| APA | Wei, Z,Zou, XL,Wang, HZ,Tei, JG,Wu, Y,&Liao, I.(2015).The N-terminal leucine-zipper motif in PTRF/cavin-1 is essential and sufficient for its caveolae-association.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,456(3),750-756. |
| MLA | Wei, Z,et al."The N-terminal leucine-zipper motif in PTRF/cavin-1 is essential and sufficient for its caveolae-association".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 456.3(2015):750-756. |
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