Acetylproteomic Analysis Reveals Functional Implications of Lysine Acetylation in Human Spermatozoa (sperm)
文献类型：期刊论文
| 作者 | Yu, HG; Diao, H; Wang, CM; Lin, Y; Yu, FD; Lu, H; Xu, W; Li, Z; Shi, HJ; Zhao, SM |
| 刊名 | MOLECULAR & CELLULAR PROTEOMICS
 |
| 出版日期 | 2015 |
| 卷号 | 14期号:4页码:1009-1023 |
| 通讯作者 | Zhao, SM (reprint author), Fudan Univ, Sch Life Sci, Mol & Cell Biol Lab, 138 Med Coll St, Shanghai 200032, Peoples R China.,zhaosm@fudan.edu.cn ; zhouych@sibs.ac.cn ; ylzhang@sibcb.ac.cn |
| 英文摘要 | Male infertility is a medical condition that has been on the rise globally. Lysine acetylation of human sperm, an essential posttranslational modification involved in the etiology of sperm abnormality, is not fully understood. Therefore, we first generated a qualified pan-anti-acetyllysine monoclonal antibody to characterize the global lysine acetylation of uncapacitated normal human sperm with a proteomics approach. With high enrichment ratios that were up to 31%, 973 lysine-acetylated sites that matched to 456 human sperm proteins, including 671 novel lysine acetylation sites and 205 novel lysine-acetylated proteins, were identified. These proteins exhibited conserved motifs XXXKYXXX, XXXKFXXX, and XXXKHXXX, were annotated to function in multiple metabolic processes, and were localized predominantly in the mitochondrion and cytoplasmic fractions. Between the uncapacitated and capacitated sperm, different acetylation profiles in regard to functional proteins involved in sperm capacitation, sperm-egg recognition, sperm-egg plasma fusion, and fertilization were observed, indicating that acetylation of functional proteins may be required during sperm capacitation. Bioinformatics analysis revealed association of acetylated proteins with diseases and drugs. Novel acetylation of voltage-dependent anion channel proteins was also found. With clinical sperm samples, we observed differed lysine acetyltransferases and lysine deacetylases expression between normal sperm and abnormal sperm of asthenospermia or necrospermia. Furthermore, with sperm samples impaired by epigallocatechin gallate to mimic asthenospermia, we observed that inhibition of sperm motility was partly through the blockade of voltage-dependent anion channel 2 Lys-74 acetylation combined with reduced ATP levels and mitochondrial membrane potential. Taken together, we obtained a qualified pananti-acetyllysine monoclonal antibody, analyzed the acetylproteome of uncapacitated human sperm, and revealed associations between functional protein acetylation and sperm functions. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemical Research Methods |
| 关键词[WOS] | HISTONE DEACETYLASE INHIBITORS ; POSTTRANSLATIONAL MODIFICATIONS ; MALE-INFERTILITY ; MALE-FERTILITY ; ALPHA-TUBULIN ; PROTEOMICS ; GENOME ; GENE ; IDENTIFICATION ; CELLS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000352194400016 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/52]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Yu, HG,Diao, H,Wang, CM,et al. Acetylproteomic Analysis Reveals Functional Implications of Lysine Acetylation in Human Spermatozoa (sperm)[J]. MOLECULAR & CELLULAR PROTEOMICS,2015,14(4):1009-1023. |
| APA | Yu, HG.,Diao, H.,Wang, CM.,Lin, Y.,Yu, FD.,...&Zhang, YL.(2015).Acetylproteomic Analysis Reveals Functional Implications of Lysine Acetylation in Human Spermatozoa (sperm).MOLECULAR & CELLULAR PROTEOMICS,14(4),1009-1023. |
| MLA | Yu, HG,et al."Acetylproteomic Analysis Reveals Functional Implications of Lysine Acetylation in Human Spermatozoa (sperm)".MOLECULAR & CELLULAR PROTEOMICS 14.4(2015):1009-1023. |
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