Combination of inflammation-related cytokines promotes long-term muscle stem cell expansion
文献类型：期刊论文
| 作者 | Fu, X; Xiao, J; Wei, YN; Li, S; Liu, Y; Yin, J; Sun, K; Sun, H; Wang, HT; Zhang, ZK |
| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2015 |
| 卷号 | 25期号:6页码:655-673 |
| 关键词 | muscle stem cells T cells muscle regeneration inflammation long-term expansion stemness maintenance |
| 通讯作者 | Hu, P (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Cell Biol, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,hongyanwang@sibcb.ac.cn ; hup@sibcb.ac.cn |
| 英文摘要 | Muscle stem cells (MuSCs, satellite cells) are the major contributor to muscle regeneration. Like most adult stem cells, long-term expansion of MuSCs in vitro is difficult. The in vivo muscle regeneration abilities of MuSCs are quickly lost after culturing in vitro, which prevents the potential applications of MuSCs in cell-based therapies. Here, we establish a system to serially expand MuSCs in vitro for over 20 passages by mimicking the endogenous microenvironment. We identified that the combination of four pro-inflammatory cytokines, IL-1 alpha, IL-13, TNF-alpha, and IFN-gamma, secreted by T cells was able to stimulate MuSC proliferation in vivo upon injury and promote serial expansion of MuSCs in vitro. The expanded MuSCs can replenish the endogenous stem cell pool and are capable of repairing multiple rounds of muscle injuries in vivo after a single transplantation. The establishment of the in vitro system provides us a powerful method to expand functional MuSCs to repair muscle injuries. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | REGULATORY T-CELLS ; SKELETAL-MUSCLE ; SATELLITE CELL ; SELF-RENEWAL ; MAINTAIN QUIESCENCE ; MUSCULAR-DYSTROPHY ; SECRETORY ORGAN ; GENE-EXPRESSION ; REGENERATION ; ACTIVATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000355554800005 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/72]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Fu, X,Xiao, J,Wei, YN,et al. Combination of inflammation-related cytokines promotes long-term muscle stem cell expansion[J]. CELL RESEARCH,2015,25(6):655-673. |
| APA | Fu, X.,Xiao, J.,Wei, YN.,Li, S.,Liu, Y.,...&Hu, P.(2015).Combination of inflammation-related cytokines promotes long-term muscle stem cell expansion.CELL RESEARCH,25(6),655-673. |
| MLA | Fu, X,et al."Combination of inflammation-related cytokines promotes long-term muscle stem cell expansion".CELL RESEARCH 25.6(2015):655-673. |
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