Anchorage-dependent binding of integrin I-domain to adhesion ligands
文献类型：期刊论文
| 作者 | Fu, X; Xu, Y; Wu, CY; Moy, VT; Zhang, XF |
| 刊名 | JOURNAL OF MOLECULAR RECOGNITION
 |
| 出版日期 | 2015 |
| 卷号 | 28期号:6页码:385-392 |
| 关键词 | integrin atomic force microscopy cell adhesion single-molecule force spectroscopy |
| 通讯作者 | Zhang, XF (reprint author), Lehigh Univ, Bioengn Program, 19 Mem Dr West, Bethlehem, PA 18015 USA.,xiz310@lehigh.edu |
| 英文摘要 | The dynamic interactions between leukocyte integrin receptors and ligands in the vascular endothelium, extracellular matrix, or invading pathogens result in leukocyte adhesion, extravasation, and phagocytosis. This work examined the mechanical strength of the connection between iC3b, a complement component that stimulates phagocytosis, and the ligand-binding domain, the I-domain, of integrin (M2). Single-molecule force measurements of (M) I-domain-iC3b complexes were conducted by atomic force microscope. Strikingly, depending on loading rates, immobilization of the I-domain via its C-terminus resulted in a 1.3-fold to 1.5-fold increase in unbinding force compared with I-domains immobilized via the N-terminus. The force spectra (unbinding force versus loading rate) of the I-domain-iC3b complexes revealed that the enhanced mechanical strength is due to a 2.4-fold increase in the lifetime of the I-domain-iC3b bond. Given the structural and functional similarity of all integrin I-domains, our result supports the existing allosteric regulatory model by which the ligand binding strength of integrin can be increased rapidly when a force is allowed to stretch the C-terminus of the I-domain. This type of mechanism may account for the rapid ligand affinity adjustment during leukocyte migration. Copyright (c) 2015 John Wiley & Sons, Ltd. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | FUNCTION-ASSOCIATED ANTIGEN-1 ; MONOCLONAL-ANTIBODY ; DYNAMIC STRENGTH ; STRUCTURAL BASIS ; INSERTED DOMAIN ; IC3B BINDING ; ACTIVATION ; CONFORMATION ; RECOGNITION ; CD11B/CD18 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000353460800006 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/76]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Fu, X,Xu, Y,Wu, CY,et al. Anchorage-dependent binding of integrin I-domain to adhesion ligands[J]. JOURNAL OF MOLECULAR RECOGNITION,2015,28(6):385-392. |
| APA | Fu, X,Xu, Y,Wu, CY,Moy, VT,&Zhang, XF.(2015).Anchorage-dependent binding of integrin I-domain to adhesion ligands.JOURNAL OF MOLECULAR RECOGNITION,28(6),385-392. |
| MLA | Fu, X,et al."Anchorage-dependent binding of integrin I-domain to adhesion ligands".JOURNAL OF MOLECULAR RECOGNITION 28.6(2015):385-392. |
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