Protein arginine methyltransferase CARM1 attenuates the paraspeckle-mediated nuclear retention of mRNAs containing IRAlus
文献类型：期刊论文
| 作者 | Hu, SB; Xiang, JF; Li, X; Xu, YF; Xue, W; Huang, M; Wong, CC; Sagum, CA; Bedford, MT; Yang, L |
| 刊名 | GENES & DEVELOPMENT
 |
| 出版日期 | 2015 |
| 卷号 | 29期号:6页码:630-645 |
| 关键词 | paraspeckles p54(nrb) nuclear retention CARM1 NEAT1 |
| 通讯作者 | Yang, L (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, German Max Planck Soc MPG Partner Inst Computat, Key Lab Computat Biol, Shanghai 200031, Peoples R China.,linglingchen@sibcb.ac.cn ; dcheng@mdanderson.org |
| 英文摘要 | In many cells, mRNAs containing inverted repeated Alu elements (IRAlus) in their 3' untranslated regions (UTRs) are inefficiently exported to the cytoplasm. Such nuclear retention correlates with paraspeckle-associated protein complexes containing p54(nrb). However, nuclear retention of mRNAs containing IRAlus is variable, and how regulation of retention and export is achieved is poorly understood. Here we show one mechanism of such regulation via the arginine methyltransferase CARM1 (coactivator-associated arginine methyltransferase 1). We demonstrate that disruption of CARM1 enhances the nuclear retention of mRNAs containing IRAlus. CARM1 regulates this nuclear retention pathway at two levels: CARM1 methylates the coiled-coil domain of p54nrb, resulting in reduced binding of p54nrb to mRNAs containing IRAlus, and also acts as a transcription regulator to suppress NEAT1 transcription, leading to reduced paraspeckle formation. These actions of CARM1 work together synergistically to regulate the export of transcripts containing IRAlus from paraspeckles under certain cellular stresses, such as poly(I:C) treatment. This work demonstrates how a post-translational modification of an RNA-binding protein affects protein-RNA interaction and also uncovers a mechanism of transcriptional regulation of the long noncoding RNA NEAT1. |
| 学科主题 | Cell Biology; Developmental Biology; Genetics & Heredity |
| 类目[WOS] | Cell Biology ; Developmental Biology ; Genetics & Heredity |
| 关键词[WOS] | LONG NONCODING RNA ; INVERTED REPEATS ; GENE-EXPRESSION ; BINDING PROTEIN ; METHYLATION ; TRANSCRIPTION ; BODIES ; DIFFERENTIATION ; ACTIVATION ; RELOCATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000351556700006 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/77]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Hu, SB,Xiang, JF,Li, X,et al. Protein arginine methyltransferase CARM1 attenuates the paraspeckle-mediated nuclear retention of mRNAs containing IRAlus[J]. GENES & DEVELOPMENT,2015,29(6):630-645. |
| APA | Hu, SB.,Xiang, JF.,Li, X.,Xu, YF.,Xue, W.,...&Chen, LL.(2015).Protein arginine methyltransferase CARM1 attenuates the paraspeckle-mediated nuclear retention of mRNAs containing IRAlus.GENES & DEVELOPMENT,29(6),630-645. |
| MLA | Hu, SB,et al."Protein arginine methyltransferase CARM1 attenuates the paraspeckle-mediated nuclear retention of mRNAs containing IRAlus".GENES & DEVELOPMENT 29.6(2015):630-645. |
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