Mechanistic insights into EGFR membrane clustering revealed by super-resolution imaging
文献类型：期刊论文
| 作者 | Gao, J; Wang, Y; Cai, MJ; Pan, YG; Xu, HJ; Jiang, JG; Ji, HB; Wang, HD |
| 刊名 | NANOSCALE
 |
| 出版日期 | 2015 |
| 卷号 | 7期号:6页码:2511-2519 |
| 通讯作者 | Ji, HB (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Cell Biol, Shanghai 200031, Peoples R China.,hbji@sibcb.ac.cn ; hdwang@ciac.ac.cn |
| 英文摘要 | The clustering of membrane receptors such as EGFR is critical for various biological processes, for example cell signaling and tumorigenesis. However, the mechanism involved remains poorly understood. Here, we used a super resolution imaging technique, which has shattered the longstanding resolution barrier of light diffraction, to investigate the distribution of membrane EGFR on apical or basal surfaces of COS-7 cells and on the surface of suspended COS-7 cells. Our data show that more and larger EGFR clusters are detected on the apical surface in comparison with those on the basal surface and this difference is not affected by the EGFR activation state, whereas suspended COS-7 cells exhibit a moderate clustering state and a homogeneous distribution pattern, indicating that the external environment surrounding the cell membrane is the decisive factor in the EGFR clustering pattern. A dual-color dSTORM image reveals the significant colocalization of EGFR and lipid rafts; interestingly M beta CD treatment leads to a dramatic decrease of the amount and size of EGFR clusters on both apical and basal surfaces, highlighting a key role of lipid rafts in EGFR cluster formation. Altogether, our results illustrate the distribution pattern of EGFR in polarized cells and uncover the essential role of lipid rafts in EGFR cluster maintenance. |
| 学科主题 | Chemistry; Science & Technology - Other Topics; Materials Science; Physics |
| 类目[WOS] | Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied |
| 关键词[WOS] | GROWTH-FACTOR RECEPTOR ; PLASMA-MEMBRANE ; LIPID RAFTS ; LOCALIZATION MICROSCOPY ; ORGANIC FLUOROPHORES ; DIFFRACTION-LIMIT ; NANOSCALE ; ACTIVATION ; INTERNALIZATION ; COLOCALIZATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000349472300042 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/78]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Gao, J,Wang, Y,Cai, MJ,et al. Mechanistic insights into EGFR membrane clustering revealed by super-resolution imaging[J]. NANOSCALE,2015,7(6):2511-2519. |
| APA | Gao, J.,Wang, Y.,Cai, MJ.,Pan, YG.,Xu, HJ.,...&Wang, HD.(2015).Mechanistic insights into EGFR membrane clustering revealed by super-resolution imaging.NANOSCALE,7(6),2511-2519. |
| MLA | Gao, J,et al."Mechanistic insights into EGFR membrane clustering revealed by super-resolution imaging".NANOSCALE 7.6(2015):2511-2519. |
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