Generation of embryonic stem cells from mouse adipose-tissue derived cells via somatic cell nuclear transfer
文献类型：期刊论文
| 作者 | Qin, YR; Qin, JL; Zhou, CK; Li, JS; Gao, WQ |
| 刊名 | CELL CYCLE
 |
| 出版日期 | 2015 |
| 卷号 | 14期号:8页码:1282-1290 |
| 关键词 | adipose-tissue derived cells embryonic stem cells somatic cell nuclear transfer |
| 通讯作者 | Gao, WQ (reprint author), Shanghai Jiao Tong Univ, State Key Lab Oncogenes & Related Genes, Renji MedX Stem Cell Res Ctr, Ren Ji Hosp,Sch Med, Shanghai 200030, Peoples R China.,gao.weiqiang@sjtu.edu.cn |
| 英文摘要 | Somatic cells can be reprogrammed into embryonic stem cells (ESCs) by nuclear transfer (NT-ESCs), or into induced pluripotent stem cells (iPSCs) by the "Yamanaka method." However, recent studies have indicated that mouse and human iPSCs are prone to epigenetic and transcriptional aberrations, and that NT-ESCs correspond more closely to ESCs derived from in vitro fertilized embryos than iPSCs. In addition, the procedure of NT-ESCs does not involve gene modification. Demonstration of generation of NT-ESCs using an easily-accessible source of adult cell types would be very important. Adipose tissue is a source of readily accessible donor cells and can be isolated from both males and females at different ages. Here we report that NT-ESCs can be generated from adipose tissue-derived cells (ADCs). At morphological, mRNA and protein levels, these NT-ESCs show classic ESC colonies, exhibit alkaline phosphatase (AP) activity, and display normal diploid karyotypes. Importantly, these cells express pluripotent markers including Oct4, Sox2, Nanog and SSEA-1. Furthermore, they can differentiate in vivo into various types of cells from 3 germinal layers by teratoma formation assays. This study demonstrates for the first time that ESCs can be generated from the adipose tissue by somatic cell nuclear transfer (SCNT) and suggests that ADCs can be a new donor-cell type for potential therapeutic cloning. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | PLURIPOTENT CELLS ; DNA METHYLATION ; COPY NUMBER ; MICE ; IDENTIFICATION ; MEMORY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000353168600024 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/86]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Qin, YR,Qin, JL,Zhou, CK,et al. Generation of embryonic stem cells from mouse adipose-tissue derived cells via somatic cell nuclear transfer[J]. CELL CYCLE,2015,14(8):1282-1290. |
| APA | Qin, YR,Qin, JL,Zhou, CK,Li, JS,&Gao, WQ.(2015).Generation of embryonic stem cells from mouse adipose-tissue derived cells via somatic cell nuclear transfer.CELL CYCLE,14(8),1282-1290. |
| MLA | Qin, YR,et al."Generation of embryonic stem cells from mouse adipose-tissue derived cells via somatic cell nuclear transfer".CELL CYCLE 14.8(2015):1282-1290. |
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