pH-dependent recognition of apoptotic and necrotic cells by the human dendritic cell receptor DEC205
文献类型：期刊论文
| 作者 | Cao, LX; Shi, XY; Chang, HS; Zhang, QF; He, YN |
| 刊名 | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
 |
| 出版日期 | 2015 |
| 卷号 | 112期号:23页码:7237-7242 |
| 关键词 | DEC205 pH dependence apoptosis cryoEM mannose receptor family |
| 通讯作者 | He, YN (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Natl Ctr Prot Sci,State Key Lab Mol Biol, Shanghai 201210, Peoples R China.,he@sibcb.ac.cn |
| 英文摘要 | Dendritic cells play important roles in regulating innate and adaptive immune responses. DEC205 (CD205) is one of the major endocytotic receptors on dendritic cells and has been widely used for vaccine generation against viruses and tumors. However, little is known about its structure and functional mechanism. Here we determine the structure of the human DEC205 ectodomain by cryoelectron microscopy. The structure shows that the 12 extracellular domains form a compact double ring-shaped conformation at acidic pH and become extended at basic pH. Biochemical data indicate that the pH-dependent conformational change of DEC205 is correlated with ligand binding and release. DEC205 only binds to apoptotic and necrotic cells at acidic pH, whereas live cells cannot be recognized by DEC205 at either acidic or basic conditions. These results suggest that DEC205 is an immune receptor that recognizes apoptotic and necrotic cells specifically through a pH-dependent mechanism. |
| 学科主题 | Science & Technology - Other Topics |
| 类目[WOS] | Multidisciplinary Sciences |
| 关键词[WOS] | MANNOSE RECEPTOR ; IMMUNE-RESPONSES ; IN-VIVO ; EXTRACELLULAR ACIDOSIS ; ELECTRON-MICROSCOPY ; CLEARANCE ; DEC-205 ; INNATE ; PHAGOCYTOSIS ; ANTIGENS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000355823200047 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/101]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Cao, LX,Shi, XY,Chang, HS,et al. pH-dependent recognition of apoptotic and necrotic cells by the human dendritic cell receptor DEC205[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2015,112(23):7237-7242. |
| APA | Cao, LX,Shi, XY,Chang, HS,Zhang, QF,&He, YN.(2015).pH-dependent recognition of apoptotic and necrotic cells by the human dendritic cell receptor DEC205.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,112(23),7237-7242. |
| MLA | Cao, LX,et al."pH-dependent recognition of apoptotic and necrotic cells by the human dendritic cell receptor DEC205".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 112.23(2015):7237-7242. |
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