A G-quadruplex DNA structure resolvase, RHAU, is essential for spermatogonia differentiation
文献类型：期刊论文
| 作者 | Gao, X; Ma, W; Nie, J; Zhang, C; Zhang, J; Yao, G; Han, J; Xu, J; Hu, B; Du, Y |
| 刊名 | CELL DEATH & DISEASE
 |
| 出版日期 | 2015 |
| 卷号 | 6期号:1页码:e1610-e1610 |
| 通讯作者 | Zhang, Y (reprint author), Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai Key Lab Mol Androl, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,zhongzhouyang@mail.nju.edu.cn ; xingxuhuang@mail.nju.edu.cn ; ylzhang@sibcb.ac.cn |
| 英文摘要 | G-quadruplex (G4) DNA and G4 DNA resolvase are involved in a variety of biological processes. To understand the biological function of G4 DNA structures and their resolvases in spermatogenesis, we investigated the distribution of G4 structures in mouse testis and identified their alterations during spermatogenesis. Meanwhile, we studied the function of RNA helicase associated with AU-rich element (RHAU), a G4 DNA resolvase, in spermatogenesis with a germ-cell-specific knockout mouse model. The results showed that the ablation of RHAU in germ cells caused the increase of G4 structures and thus resulted in the decrease of spermatogonial differentiation. c-kit, a spermatogonia differentiation-related gene, contains two G4 DNA motifs on its promoter. We found its expression was significantly downregulated in RHAU conditional knockout testis. A further analysis demonstrated that RHAU directly bound to the G4 structures to activate c-kit expression. We concluded that RHAU regulates spermatogonia differentiation by promoting c-kit expression via directly binding to the G4 DNA motifs c-kit promoter. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | DOUBLE-STRAND BREAKS ; CELL SELF-RENEWAL ; RNA HELICASE RHAU ; C-KIT ONCOGENE ; TETRAMOLECULAR QUADRUPLEX ; PROMOTER REGION ; GENE-EXPRESSION ; REGULATED GENES ; SMALL-MOLECULE ; MAJOR SOURCE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000349020500029 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/114]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Gao, X,Ma, W,Nie, J,et al. A G-quadruplex DNA structure resolvase, RHAU, is essential for spermatogonia differentiation[J]. CELL DEATH & DISEASE,2015,6(1):e1610-e1610. |
| APA | Gao, X.,Ma, W.,Nie, J.,Zhang, C.,Zhang, J.,...&Zhang, Y.(2015).A G-quadruplex DNA structure resolvase, RHAU, is essential for spermatogonia differentiation.CELL DEATH & DISEASE,6(1),e1610-e1610. |
| MLA | Gao, X,et al."A G-quadruplex DNA structure resolvase, RHAU, is essential for spermatogonia differentiation".CELL DEATH & DISEASE 6.1(2015):e1610-e1610. |
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