A single NS2 mutation of K86R promotes PR8 vaccine donor virus growth in Vero cells
文献类型：期刊论文
| 作者 | Zhang, H; Han, QL; Ping, XQ; Li, L; Chang, C; Chen, Z; Shu, YL; Xu, K; Sun, B |
| 刊名 | VIROLOGY
 |
| 出版日期 | 2015 |
| 卷号 | 482期号:1页码:32-40 |
| 关键词 | Influenza A virus Vaccine donor virus Vero cells NS2 |
| 通讯作者 | Sun, B (reprint author), Chinese Acad Sci, Inst Pasteur Shanghai, Shanghai Inst Biol Sci, Key Lab Mol Virol & Immunol, 320 YueYang Rd, Shanghai 200031, Peoples R China.,kxu@sibs.ac.cn |
| 英文摘要 | Vaccination is the most effective way to prevent and control infection by influenza viruses, and a cell-culture-based vaccine production system is preferred as the future choice for the large-scale production of influenza vaccines. As one of the WHO-recommended cell lines for producing influenza vaccines, Vero cells do not efficiently support the growth of the current influenza A virus vaccine donor strain, the A/Puerto Rico/8/1934 (PR8) virus. In this study, a single mutation of K86R in the NS2 protein can sufficiently render the high-yielding property to the PR8 virus in Vero cells. Further analysis showed that the later steps in the virus replication cycle were accelerated by NS2(K86R) mutation, which may relate to an enhanced interaction between NS2(K86R) and the components of host factor F1Fo-ATPase, FoB and F1 beta. Because the NS2(K86R) mutation does not increase PR8 virulence in either mice or embryonated eggs, the PR8-NS2(K86R) virus could serve as a promising vaccine donor strain in Vero cells. (C) 2015 Elsevier Inc. All rights reserved. |
| 学科主题 | Virology |
| 类目[WOS] | Virology |
| 关键词[WOS] | INFLUENZA-A-VIRUS ; MDCK CELLS ; CULTURE ; PROTEIN ; REPLICATION ; SYSTEM ; MODEL ; CULTIVATION ; GENERATION ; INFECTION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000356401900005 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/115]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhang, H,Han, QL,Ping, XQ,et al. A single NS2 mutation of K86R promotes PR8 vaccine donor virus growth in Vero cells[J]. VIROLOGY,2015,482(1):32-40. |
| APA | Zhang, H.,Han, QL.,Ping, XQ.,Li, L.,Chang, C.,...&Sun, B.(2015).A single NS2 mutation of K86R promotes PR8 vaccine donor virus growth in Vero cells.VIROLOGY,482(1),32-40. |
| MLA | Zhang, H,et al."A single NS2 mutation of K86R promotes PR8 vaccine donor virus growth in Vero cells".VIROLOGY 482.1(2015):32-40. |
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