Yeast Hmt1 catalyses asymmetric dimethylation of histone H3 arginine 2 in vitro
文献类型：期刊论文
| 作者 | Li, HT; Gong, T; Zhou, Z; Liu, YT; Cao, XW; He, YN; Chen, CD; Zhou, JQ |
| 刊名 | BIOCHEMICAL JOURNAL
 |
| 出版日期 | 2015 |
| 卷号 | 467期号:1页码:507-515 |
| 关键词 | arginine methyltransferase dimer Hmt1 histone H3R2 yeast |
| 通讯作者 | Chen, CD (reprint author), Univ Chinese Acad Sci, Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, State Key Lab Mol Biol,Shanghai Inst Biol Sci, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,cdchen@sibcb.ac.cn ; jqzhou@sibcb.ac.cn |
| 英文摘要 | Protein arginine methyltransferases (PRMTs) are a family of enzymes that can methylate protein arginine residues. PRMTs' substrates include histones and a variety of non-histone proteins. Previous studies have shown that yeast Hmt1 is a type I PRMT and methylates histone H4 arginine 3 and several mRNA-binding proteins. Hmt1 forms dimers or oligomers, but how dimerization or oligomerization affects its activity remains largely unknown. We now report that Hmt1 can methylate histone H3 arginine 2 (H3R2) in vitro. The dimerization but not hexamerization is essential for Hmt1's activity. Interestingly, the methyltransferase activity of Hmt1 on histone H3R2 requires reciprocal contributions from two Hmt1 molecules. Our results suggest an intermolecular trans-complementary mechanism by which Hmt1 dimer methylates its substrates. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | SACCHAROMYCES-CEREVISIAE ; GENE-EXPRESSION ; CRYSTAL-STRUCTURE ; METHYLATION ; METHYLTRANSFERASE ; CHROMATIN ; PROTEINS ; INSIGHTS ; DNA ; TRIMETHYLATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000353217200013 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/116]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Li, HT,Gong, T,Zhou, Z,et al. Yeast Hmt1 catalyses asymmetric dimethylation of histone H3 arginine 2 in vitro[J]. BIOCHEMICAL JOURNAL,2015,467(1):507-515. |
| APA | Li, HT.,Gong, T.,Zhou, Z.,Liu, YT.,Cao, XW.,...&Zhou, JQ.(2015).Yeast Hmt1 catalyses asymmetric dimethylation of histone H3 arginine 2 in vitro.BIOCHEMICAL JOURNAL,467(1),507-515. |
| MLA | Li, HT,et al."Yeast Hmt1 catalyses asymmetric dimethylation of histone H3 arginine 2 in vitro".BIOCHEMICAL JOURNAL 467.1(2015):507-515. |
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