Soluble fusion expression, characterization and localization of human beta-defensin 6
文献类型：期刊论文
| 作者 | Xin, AJ; Zhao, Y; Yu, HG; Shi, HJ; Liu, HY; Diao, H; Zhang, YL |
| 刊名 | MOLECULAR MEDICINE REPORTS
 |
| 出版日期 | 2014 |
| 卷号 | 9期号:1页码:149-155 |
| 关键词 | human beta-defensin 6 antimicrobial peptide intein purification binding with heparin and lipopolysaccharide |
| 通讯作者 | Zhang, YL (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai Key Lab Mol Androl,State Key Lab Mol Bio, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,diaohua@gmail.com ; ylzhang@sibs.ac.cn |
| 英文摘要 | Human -defensin 6 (DEFB106) is an antimicrobial peptide expressed in the epididymis, testis and lung, which indicates that DEFB106 may be involved in innate immunity and fertility. However, as a -defensin, this protein has not been well characterized. Using an intein-mediated fusion expression system, the recombinant DEFB106 was expressed and purified (yield, 3-5 mg/l) under optimized conditions. The purified protein was characterized using mass spectrometry and circular dichroism spectroscopy. The measured molecular weight was consistent with its theoretical value and the predominant secondary structure was -sheet, the common structure of -defensin family members. The purified DEFB106 showed antimicrobial activity against not only Escherichia coli (E. coli) and Candida albicans (C. albicans) SC5314, but also Staphylococcus aureus (S. aureus) CMCC26003. Furthermore, it exhibited a high affinity for heparin and lipopolysaccharide. In addition, it was determined that native DEFB106 was located in the epididymis, bone marrow and skin. These observations may aid in the determination of the physiological and pathological functions of DEFB106. |
| 学科主题 | Oncology; Research & Experimental Medicine |
| 类目[WOS] | Oncology ; Medicine, Research & Experimental |
| 关键词[WOS] | ESCHERICHIA-COLI ; 2 HBD2 ; PEPTIDES ; BINDING ; IDENTIFICATION ; EVOLUTION ; MEMBRANES ; INSERTION ; LIGATION ; PROTEINS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000330783700024 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/129]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Xin, AJ,Zhao, Y,Yu, HG,et al. Soluble fusion expression, characterization and localization of human beta-defensin 6[J]. MOLECULAR MEDICINE REPORTS,2014,9(1):149-155. |
| APA | Xin, AJ.,Zhao, Y.,Yu, HG.,Shi, HJ.,Liu, HY.,...&Zhang, YL.(2014).Soluble fusion expression, characterization and localization of human beta-defensin 6.MOLECULAR MEDICINE REPORTS,9(1),149-155. |
| MLA | Xin, AJ,et al."Soluble fusion expression, characterization and localization of human beta-defensin 6".MOLECULAR MEDICINE REPORTS 9.1(2014):149-155. |
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