热门
5mC Oxidation by Tet2 Modulates Enhancer Activity and Timing of Transcriptome Reprogramming during Differentiation
文献类型：期刊论文
| 作者 | Hon, GC; Song, CX; Du, TT; Jin, FL; Selvaraj, S; Lee, AY; Yen, CA; Ye, Z; Mao, SQ; Wang, BA |
| 刊名 | MOLECULAR CELL
 |
| 出版日期 | 2014 |
| 卷号 | 56期号:2页码:286-297 |
| 通讯作者 | He, C (reprint author), Univ Chicago, Howard Hughes Med Inst, Dept Chem, 5841 S Maryland Ave, Chicago, IL 60637 USA.,chuanhe@uchicago.edu ; biren@ucsd.edu |
| 英文摘要 | In mammals, cytosine methylation (5mC) is widely distributed throughout the genome but is notably depleted from active promoters and enhancers. While the role of DNA methylation in promoter silencing has been well documented, the function of this epigenetic mark at enhancers remains unclear. Recent experiments have demonstrated that enhancers are enriched for 5-hydroxymethylcytosine (5hmC), an oxidization product of the Tet family of 5mC dioxygenases and an intermediate of DNA demethylation. These results support the involvement of Tet proteins in the regulation of dynamic DNA methylation at enhancers. By mapping DNA methylation and hydroxymethylation at base resolution, we find that deletion of Tet2 causes extensive loss of 5hmC at enhancers, accompanied by enhancer hypermethylation, reduction of enhancer activity, and delayed gene induction in the early steps of differentiation. Our results reveal that DNA demethylation modulates enhancer activity, and its disruption influences the timing of transcriptome reprogramming during cellular differentiation. |
| 学科主题 | Biochemistry & Molecular Biology; Cell Biology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Cell Biology |
| 关键词[WOS] | EMBRYONIC STEM-CELLS ; DNA METHYLATION ; MAMMALIAN DEVELOPMENT ; GENE-EXPRESSION ; REGULATORY SEQUENCES ; HUMAN GENOME ; ES CELLS ; 5-HYDROXYMETHYLCYTOSINE ; CHROMATIN ; ROLES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000344484600010 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/138]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Hon, GC,Song, CX,Du, TT,et al. 5mC Oxidation by Tet2 Modulates Enhancer Activity and Timing of Transcriptome Reprogramming during Differentiation[J]. MOLECULAR CELL,2014,56(2):286-297. |
| APA | Hon, GC.,Song, CX.,Du, TT.,Jin, FL.,Selvaraj, S.,...&Ren, B.(2014).5mC Oxidation by Tet2 Modulates Enhancer Activity and Timing of Transcriptome Reprogramming during Differentiation.MOLECULAR CELL,56(2),286-297. |
| MLA | Hon, GC,et al."5mC Oxidation by Tet2 Modulates Enhancer Activity and Timing of Transcriptome Reprogramming during Differentiation".MOLECULAR CELL 56.2(2014):286-297. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。