A convenient system for highly specific and sensitive detection of miRNA expression
文献类型：期刊论文
| 作者 | Li, XQ; Ni, MJ; Zhang, CB; Ma, WB; Zhang, YL |
| 刊名 | RNA-A PUBLICATION OF THE RNA SOCIETY
 |
| 出版日期 | 2014 |
| 卷号 | 20期号:2页码:252-259 |
| 关键词 | miRNA small RNA liquid hybridization avidin-biotin detection system PCR color development |
| 通讯作者 | Li, XQ (reprint author), Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai Key Lab Mol Androl, Shanghai 200031, Peoples R China.,lixq@sibs.ac.cn ; ylzhang@sibs.ac.cn |
| 英文摘要 | Since the first miRNA was discovered in 1993, miRNAs have become a hotspot for biological research. In order to feed this demand, a robust method is required to detect miRNA gene expression. Development of a detection method is more difficult for miRNAs than for long RNAs, such as mRNA, owing to their small size. Existing methods have limitations; thus, new methods are required. We describe a new system for detecting miRNA expression, which can distinguish miRNA from its precursor and has single-nucleotide resolution. It has single molecule and multiplex detection potential. It may be performed as a polymerase chain reaction (PCR) method, a blotting method, or a macroarray method according to the analyst's preference. This personalized system provides a convenient tool for the detection of miRNA gene expression. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | MICRORNA RESEARCH ; SMALL RNAS ; QUANTIFICATION ; HYBRIDIZATION ; PCR |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000329850500011 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/150]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Li, XQ,Ni, MJ,Zhang, CB,et al. A convenient system for highly specific and sensitive detection of miRNA expression[J]. RNA-A PUBLICATION OF THE RNA SOCIETY,2014,20(2):252-259. |
| APA | Li, XQ,Ni, MJ,Zhang, CB,Ma, WB,&Zhang, YL.(2014).A convenient system for highly specific and sensitive detection of miRNA expression.RNA-A PUBLICATION OF THE RNA SOCIETY,20(2),252-259. |
| MLA | Li, XQ,et al."A convenient system for highly specific and sensitive detection of miRNA expression".RNA-A PUBLICATION OF THE RNA SOCIETY 20.2(2014):252-259. |
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