热门
Direct Reprogramming of Human Fibroblasts to Functional and Expandable Hepatocytes
文献类型：期刊论文
| 作者 | Huang, PY; Zhang, LD; Gao, YM; He, ZY; Yao, D; Wu, ZT; Cen, J; Chen, XT; Liu, CC; Hu, YP |
| 刊名 | CELL STEM CELL
 |
| 出版日期 | 2014 |
| 卷号 | 14期号:3页码:370-384 |
| 通讯作者 | Hui, LJ (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Cell Biol, Shanghai 200031, Peoples R China.,ljhui@sibcb.ac.cn |
| 英文摘要 | The generation of large numbers of functional human hepatocytes for cell-based approaches to liver disease is an important and unmet goal. Direct reprogramming of fibroblasts to hepatic lineages could offer a solution to this problem but so far has only been achieved with mouse cells. Here, we generated human induced hepatocytes (hiHeps) from fibroblasts by lentiviral expression of FOXA3, HNF1A, and HNF4A. hiHeps express hepatic gene programs, can be expanded in vitro, and display functions characteristic of mature hepatocytes, including cytochrome P450 enzyme activity and biliary drug clearance. Upon transplantation into mice with concanavalin-A-induced acute liver failure and fatal metabolic liver disease due to fumarylacetoacetate dehydrolase (Fah) deficiency, hiHeps restore the liver function and prolong survival. Collectively, our results demonstrate successful lineage conversion of nonhepatic human cells into mature hepatocytes with potential for biomedical and pharmaceutical applications. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell & Tissue Engineering ; Cell Biology |
| 关键词[WOS] | BIOARTIFICIAL LIVER ; DRUG-METABOLISM ; TRANSCRIPTION FACTORS ; P53-DEFICIENT MICE ; IN-VIVO ; CELLS ; CONVERSION ; CHALLENGES ; INDUCTION ; FAILURE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000332533400013 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/158]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Huang, PY,Zhang, LD,Gao, YM,et al. Direct Reprogramming of Human Fibroblasts to Functional and Expandable Hepatocytes[J]. CELL STEM CELL,2014,14(3):370-384. |
| APA | Huang, PY.,Zhang, LD.,Gao, YM.,He, ZY.,Yao, D.,...&Hui, LJ.(2014).Direct Reprogramming of Human Fibroblasts to Functional and Expandable Hepatocytes.CELL STEM CELL,14(3),370-384. |
| MLA | Huang, PY,et al."Direct Reprogramming of Human Fibroblasts to Functional and Expandable Hepatocytes".CELL STEM CELL 14.3(2014):370-384. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。