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Direct Reprogramming of Human Fibroblasts to Functional and Expandable Hepatocytes

文献类型:期刊论文

作者Huang, PY; Zhang, LD; Gao, YM; He, ZY; Yao, D; Wu, ZT; Cen, J; Chen, XT; Liu, CC; Hu, YP
刊名CELL STEM CELL
出版日期2014
卷号14期号:3页码:370-384
通讯作者Hui, LJ (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Cell Biol, Shanghai 200031, Peoples R China.,ljhui@sibcb.ac.cn
英文摘要The generation of large numbers of functional human hepatocytes for cell-based approaches to liver disease is an important and unmet goal. Direct reprogramming of fibroblasts to hepatic lineages could offer a solution to this problem but so far has only been achieved with mouse cells. Here, we generated human induced hepatocytes (hiHeps) from fibroblasts by lentiviral expression of FOXA3, HNF1A, and HNF4A. hiHeps express hepatic gene programs, can be expanded in vitro, and display functions characteristic of mature hepatocytes, including cytochrome P450 enzyme activity and biliary drug clearance. Upon transplantation into mice with concanavalin-A-induced acute liver failure and fatal metabolic liver disease due to fumarylacetoacetate dehydrolase (Fah) deficiency, hiHeps restore the liver function and prolong survival. Collectively, our results demonstrate successful lineage conversion of nonhepatic human cells into mature hepatocytes with potential for biomedical and pharmaceutical applications.
学科主题Cell Biology
类目[WOS]Cell & Tissue Engineering ; Cell Biology
关键词[WOS]BIOARTIFICIAL LIVER ; DRUG-METABOLISM ; TRANSCRIPTION FACTORS ; P53-DEFICIENT MICE ; IN-VIVO ; CELLS ; CONVERSION ; CHALLENGES ; INDUCTION ; FAILURE
收录类别SCI
语种英语
WOS记录号WOS:000332533400013
版本出版稿
源URL[http://202.127.25.143/handle/331003/158]  
专题上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式
GB/T 7714
Huang, PY,Zhang, LD,Gao, YM,et al. Direct Reprogramming of Human Fibroblasts to Functional and Expandable Hepatocytes[J]. CELL STEM CELL,2014,14(3):370-384.
APA Huang, PY.,Zhang, LD.,Gao, YM.,He, ZY.,Yao, D.,...&Hui, LJ.(2014).Direct Reprogramming of Human Fibroblasts to Functional and Expandable Hepatocytes.CELL STEM CELL,14(3),370-384.
MLA Huang, PY,et al."Direct Reprogramming of Human Fibroblasts to Functional and Expandable Hepatocytes".CELL STEM CELL 14.3(2014):370-384.

入库方式: OAI收割

来源:上海生物化学与细胞生物学研究所

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