A Microtubule-Associated Zinc Finger Protein, BuGZ, Regulates Mitotic Chromosome Alignment by Ensuring Bub3 Stability and Kinetochore Targeting
文献类型：期刊论文
| 作者 | Jiang, H; He, XN; Wang, SS; Jia, JL; Wan, YH; Wang, YJ; Zeng, R; Yates, J; Zhu, XL; Zheng, YX |
| 刊名 | DEVELOPMENTAL CELL
 |
| 出版日期 | 2014 |
| 卷号 | 28期号:3页码:268-281 |
| 通讯作者 | Zhu, XL (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Cell Biol, Shanghai 200031, Peoples R China.,xlzhu@sibcb.ac.cn ; zheng@ciwemb.edu |
| 英文摘要 | Equal chromosome segregation requires proper assembly of many proteins, including Bub3, onto kinetochores to promote kinetochore-microtubule interactions. By screening for mitotic regulators in the spindle envelope and matrix (Spemix), we identify a conserved Bub3 interacting and GLE-2-binding sequence (GLEBS) containing ZNF207 (BuGZ) that associates with spindle microtubules and regulates chromosome alignment. Using its conserved GLEBS, BuGZ directly binds and stabilizes Bub3. BuGZ also uses its microtubule-binding domain to enhance the loading of Bub3 to kinetochores that have assumed initial interactions with microtubules in prometaphase. This enhanced Bub3 loading is required for proper chromosome alignment and metaphase to anaphase progression. Interestingly, we show that microtubules are required for the highest kinetochore loading of Bub3, BubR1, and CENP-E during prometaphase. These findings suggest that BuGZ not only serves as a molecular chaperone for Bub3 but also enhances its loading onto kinetochores during prometaphase in a microtubule-dependent manner to promote chromosome alignment. |
| 学科主题 | Cell Biology; Developmental Biology |
| 类目[WOS] | Cell Biology ; Developmental Biology |
| 关键词[WOS] | SPINDLE-ASSEMBLY CHECKPOINT ; XENOPUS EGG EXTRACTS ; RNA EXPORT FACTOR ; CENP-E ; STRUCTURAL-ANALYSIS ; AURORA-A ; KINASE ; ATTACHMENT ; LOCALIZATION ; MITOSIS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000331592300007 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/165]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Jiang, H,He, XN,Wang, SS,et al. A Microtubule-Associated Zinc Finger Protein, BuGZ, Regulates Mitotic Chromosome Alignment by Ensuring Bub3 Stability and Kinetochore Targeting[J]. DEVELOPMENTAL CELL,2014,28(3):268-281. |
| APA | Jiang, H.,He, XN.,Wang, SS.,Jia, JL.,Wan, YH.,...&Zheng, YX.(2014).A Microtubule-Associated Zinc Finger Protein, BuGZ, Regulates Mitotic Chromosome Alignment by Ensuring Bub3 Stability and Kinetochore Targeting.DEVELOPMENTAL CELL,28(3),268-281. |
| MLA | Jiang, H,et al."A Microtubule-Associated Zinc Finger Protein, BuGZ, Regulates Mitotic Chromosome Alignment by Ensuring Bub3 Stability and Kinetochore Targeting".DEVELOPMENTAL CELL 28.3(2014):268-281. |
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