Redox-active quinones induces genome-wide DNA methylation changes by an iron-mediated and Tet-dependent mechanism
文献类型：期刊论文
| 作者 | Zhao, BL; Yang, Y; Wang, XL; Chong, ZC; Yin, RC; Song, SH; Zhao, C; Li, CP; Huang, H; Sun, BF |
| 刊名 | NUCLEIC ACIDS RESEARCH
 |
| 出版日期 | 2014 |
| 卷号 | 42期号:3页码:1593-1605 |
| 通讯作者 | Wang, HL (reprint author), Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China.,ygyang@big.ac.cn ; hlwang@rcees.ac.cn |
| 英文摘要 | DNA methylation has been proven to be a critical epigenetic mark important for various cellular processes. Here, we report that redox-active quinones, a ubiquitous class of chemicals found in natural products, cancer therapeutics and environment, stimulate the conversion of 5mC to 5hmC in vivo, and increase 5hmC in 5751 genes in cells. 5hmC increase is associated with significantly altered gene expression of 3414 genes. Interestingly, in quinone-treated cells, labile iron-sensitive protein ferritin light chain showed a significant increase at both mRNA and protein levels indicating a role of iron regulation in stimulating Tet-mediated 5mC oxidation. Consistently, the deprivation of cellular labile iron using specific chelator blocked the 5hmC increase, and a delivery of labile iron increased the 5hmC level. Moreover, both Tet1/Tet2 knockout and dimethylox-alylglycine- induced Tet inhibition diminished the 5hmC increase. These results suggest an iron-regulated Tet-dependent DNA demethylation mechanism mediated by redox-active biomolecules. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | METAL-INDEPENDENT PRODUCTION ; BURROWS-WHEELER TRANSFORM ; HYDROGEN-PEROXIDE ; HYDROXYL RADICALS ; READ ALIGNMENT ; MAMMALIAN DNA ; RNA-SEQ ; HYPOMETHYLATION ; 5-HYDROXYMETHYLCYTOSINE ; 5-METHYLCYTOSINE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000331138800021 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/167]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhao, BL,Yang, Y,Wang, XL,et al. Redox-active quinones induces genome-wide DNA methylation changes by an iron-mediated and Tet-dependent mechanism[J]. NUCLEIC ACIDS RESEARCH,2014,42(3):1593-1605. |
| APA | Zhao, BL.,Yang, Y.,Wang, XL.,Chong, ZC.,Yin, RC.,...&Wang, HL.(2014).Redox-active quinones induces genome-wide DNA methylation changes by an iron-mediated and Tet-dependent mechanism.NUCLEIC ACIDS RESEARCH,42(3),1593-1605. |
| MLA | Zhao, BL,et al."Redox-active quinones induces genome-wide DNA methylation changes by an iron-mediated and Tet-dependent mechanism".NUCLEIC ACIDS RESEARCH 42.3(2014):1593-1605. |
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