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Active and Passive Demethylation of Male and Female Pronuclear DNA in the Mammalian Zygote
文献类型：期刊论文
| 作者 | Guo, F; Li, XL; Liang, D; Li, T; Zhu, P; Guo, HS; Wu, XL; Wen, L; Gu, TP; Hu, BQ |
| 刊名 | CELL STEM CELL
 |
| 出版日期 | 2014 |
| 卷号 | 15期号:4页码:447-458 |
| 通讯作者 | Li, JS (reprint author), Chinese Acad Sci, Inst Biochem & Cell Biol, State Key Lab Cell Biol, Grp Epigenet Reprogramming, Shanghai 200031, Peoples R China.,jsli@sibcb.ac.cn ; tangfuchou@pku.edu.cn ; glxu@sibcb.ac.cn |
| 英文摘要 | The epigenomes of mammalian sperm and oocytes, characterized by gamete-specific 5-methylcytosine (5mC) patterns, are reprogrammed during early embryogenesis to establish full developmental potential. Previous studies have suggested that the paternal genome is actively demethylated in the zygote while the maternal genome undergoes subsequent passive demethylation via DNA replication during cleavage. Active demethylation is known to depend on 5mC oxidation by Tet dioxygenases and excision of oxidized bases by thymine DNA glycosylase (TDG). Here we show that both maternal and paternal genomes undergo widespread active and passive demethylation in zygotes before the first mitotic division. Passive demethylation was blocked by the replication inhibitor aphidicolin, and active demethylation was abrogated by deletion of Tet3 in both pronuclei. At actively demethylated loci, 5mCs were processed to unmodified cytosines. Surprisingly, the demethylation process was unaffected by the deletion of TDG from the zygote, suggesting the existence of other demethylation mechanisms downstream of Tet3-mediated oxidation. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell & Tissue Engineering ; Cell Biology |
| 关键词[WOS] | BASE EXCISION-REPAIR ; MOUSE PREIMPLANTATION DEVELOPMENT ; EMBRYONIC STEM-CELLS ; METHYLTRANSFERASES DNMT3A ; METHYLATION LANDSCAPE ; PATERNAL GENOME ; 5-HYDROXYMETHYLCYTOSINE ; TET ; DYNAMICS ; TDG |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000343235800011 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/170]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Guo, F,Li, XL,Liang, D,et al. Active and Passive Demethylation of Male and Female Pronuclear DNA in the Mammalian Zygote[J]. CELL STEM CELL,2014,15(4):447-458. |
| APA | Guo, F.,Li, XL.,Liang, D.,Li, T.,Zhu, P.,...&Xu, GL.(2014).Active and Passive Demethylation of Male and Female Pronuclear DNA in the Mammalian Zygote.CELL STEM CELL,15(4),447-458. |
| MLA | Guo, F,et al."Active and Passive Demethylation of Male and Female Pronuclear DNA in the Mammalian Zygote".CELL STEM CELL 15.4(2014):447-458. |
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