Production and characterization of recombinant human beta-defensin DEFB120
文献类型：期刊论文
| 作者 | Liu, HY; Yu, HG; Xin, AJ; Shi, HJ; Gu, YH; Zhang, YL; Diao, H; Lin, DH |
| 刊名 | JOURNAL OF PEPTIDE SCIENCE
 |
| 出版日期 | 2014 |
| 卷号 | 20期号:4页码:251-257 |
| 关键词 | human beta-defensin 120 recombinant expression antimicrobial activity LPS hemolytic activity |
| 通讯作者 | Diao, H (reprint author), Shanghai Inst Planned Parenthood Res, Shanghai 200032, Peoples R China.,diaohua@gmail.com ; dhlin@xmu.edu.cn |
| 英文摘要 | Public health of human beings is threatened by superbugs. Novel human beta-defensins, which contribute to host defense against pathogen invasion and innate immune protection, might be a potent natural candidate pool for new antibiotic lead screening. In the present work, we successfully expressed and purified a novel human beta-defensin, DEFB120, using the IMPACT-TWIN system in Escherichia coli and identified the purified homogeneous proteins using MALDI-TOF mass spectrometry. Then, we performed the fundamental studies on the structure and biological functions for the DEFB120 peptide. The recombinant DEFB120 peptide showed wide antimicrobial effects against E. coli, Staphylococcus aureus and Candida albicans strains without significant hemolytic activity. Furthermore, the high lipopolysaccharide (LPS)-binding affinity in vitro indicated that DEFB120 might be associated with the inhibition of LPS-induced inflammatory response. These results may pave a way for exploiting the essential physiological functions of DEFB120 and also for the development of natural antibiotic pools. Copyright (C) 2014 European Peptide Society and John Wiley & Sons, Ltd. |
| 学科主题 | Biochemistry & Molecular Biology; Chemistry |
| 类目[WOS] | Biochemistry & Molecular Biology ; Chemistry, Analytical |
| 关键词[WOS] | ANTIMICROBIAL PEPTIDE ; IN-VITRO ; ANTIBIOTICS ; EXPRESSION ; EPIDIDYMIS ; IDENTIFICATION ; RESISTANCE ; EVOLUTION ; SPERM ; VIVO |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000332973300003 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/181]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Liu, HY,Yu, HG,Xin, AJ,et al. Production and characterization of recombinant human beta-defensin DEFB120[J]. JOURNAL OF PEPTIDE SCIENCE,2014,20(4):251-257. |
| APA | Liu, HY.,Yu, HG.,Xin, AJ.,Shi, HJ.,Gu, YH.,...&Lin, DH.(2014).Production and characterization of recombinant human beta-defensin DEFB120.JOURNAL OF PEPTIDE SCIENCE,20(4),251-257. |
| MLA | Liu, HY,et al."Production and characterization of recombinant human beta-defensin DEFB120".JOURNAL OF PEPTIDE SCIENCE 20.4(2014):251-257. |
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