Lithocholic acid-induced placental tumor necrosis factor-alpha upregulation and syncytiotrophoblast cell apoptosis in intrahepatic cholestasis of pregnancy
文献类型：期刊论文
| 作者 | Du, QL; Zhang, YH; Pan, YD; Duan, T |
| 刊名 | HEPATOLOGY RESEARCH
 |
| 出版日期 | 2014 |
| 卷号 | 44期号:5页码:532-541 |
| 关键词 | bile acid tumor necrosis factor-alpha intrahepatic cholestasis of pregnancy lithocholic acid syncytiotrophoblast |
| 通讯作者 | Duan, T (reprint author), Tongji Univ, Sch Med, Dept Obstet, Shanghai Matern & Infant Hosp 1, Shanghai 200040, Peoples R China.,ydpan@sibcb.ac.cn ; qldu2006@yahoo.cn |
| 英文摘要 | Aim To investigate tumor necrosis factor (TNF)-alpha expression and its relationship with serum bile acids in placental trophoblasts from patients with intrahepatic cholestasis of pregnancy (ICP). Methods Human placenta, including normal pregnancies (n = 10) and patients with ICP (n = 10), were collected at term and subject to TNF-alpha measurements. Bile acid-induced TNF-alpha expression and cell apoptosis were evaluated in cultured syncytiotrophoblasts in vitro. Results ICP placental trophoblasts displayed apoptotic histological abnormalities. TNF-alpha levels in ICP tissue were significantly greater than those of controls as measured by quantitative polymerase chain reaction and western blot. Levels of placental TNF-alpha mRNA were positively correlated with serum bile acid concentration in ICP patients. In vitro, lithocholic acid (LCA) significantly enhanced TNF-alpha mRNA at both doses, by 2.07-fold at 15 mu m and by 3.41-fold at 30 mu m, whereas deoxycholic acid mildly increased TNF-alpha mRNA by 1.41-fold at 100 mu m only. LCA treatment produced significantly higher percentage of caspase-3 positive cells than vehicle treatment, rescuable by the addition of a TNF-alpha inhibitor, indicative of apoptosis induced by LCA-TNF-alpha pathway. Conclusion This study shows that the increase of TNF-alpha expression in placental trophoblasts is strongly associated with ICP pathology and is inducible by LCA in vitro, suggesting its potential value in the clinical prevention, diagnosis and treatment of ICP. |
| 学科主题 | Gastroenterology & Hepatology |
| 类目[WOS] | Gastroenterology & Hepatology |
| 关键词[WOS] | FETAL-RAT LIVER ; TNF-ALPHA ; BILE-ACIDS ; OXIDATIVE STRESS ; EXPRESSION ; DISEASE ; ROLES ; WOMEN ; BETA |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000334366900006 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/192]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Du, QL,Zhang, YH,Pan, YD,et al. Lithocholic acid-induced placental tumor necrosis factor-alpha upregulation and syncytiotrophoblast cell apoptosis in intrahepatic cholestasis of pregnancy[J]. HEPATOLOGY RESEARCH,2014,44(5):532-541. |
| APA | Du, QL,Zhang, YH,Pan, YD,&Duan, T.(2014).Lithocholic acid-induced placental tumor necrosis factor-alpha upregulation and syncytiotrophoblast cell apoptosis in intrahepatic cholestasis of pregnancy.HEPATOLOGY RESEARCH,44(5),532-541. |
| MLA | Du, QL,et al."Lithocholic acid-induced placental tumor necrosis factor-alpha upregulation and syncytiotrophoblast cell apoptosis in intrahepatic cholestasis of pregnancy".HEPATOLOGY RESEARCH 44.5(2014):532-541. |
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