A Study of Three Linker Peptides' Cleavage Efficiency at The Post-translational Level
文献类型：期刊论文
| 作者 | Li, HY; Zhou, ZM; Pan, Q; Yang, DM; Gu, JF; Shen, JN; Wang, SB; Zhang, KJ |
| 刊名 | PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
 |
| 出版日期 | 2014 |
| 卷号 | 41期号:6页码:558-566 |
| 关键词 | linker peptide IETD EEED F2A caspase activity |
| 通讯作者 | Zhang, KJ (reprint author), Chinese Acad Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China.,zhangkangjian@sibcb.ac.cn |
| 英文摘要 | IL-24 and Smac are the important candidate genes for the targeting multi-genes therapy of cancer. In the construction of IL-24 and Smac dual gene expression vector, a stable and efficient linker is critical. We use three different linkers such as IETD, EEED and F2A to obtain three eukaryotic expression plasmids which are pcDNA3.1 (+)-IL-24-IETD-Smac, pcDNA3.1 (+)-IL-24-EEED-Smac, pcDNA3.1 (+)IL-24-F2A-Smac, and then combined with 5-fluorouracil (5-FU) treatment to study the cleavage efficiency of the three linker peptides. The results showed that among three IL-24-linker-Smac dual gene expression vectors, the F2A linker is superior to IETD and EEED linkers. In addition, its cleavage efficiency is positively correlated with activated caspases. Moreover, the fusion protein mediated by both IETD and EEED linker also can be cleaved,but their upstream IL-24 protein with 4 additional amino acid residues derived from IETD or REED linker was detected with obvious degradation regulated by the ubiquitin-proteasome system. This study will provides a reference for the construction of dual-gene or muti-gene expression vector in cancer therapy. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | TARGETING GENE-VIROTHERAPY ; ONCOLYTIC ADENOVIRUS ; ANTITUMOR-ACTIVITY ; INDUCED APOPTOSIS ; CANCER ; XENOGRAFT ; TRAIL ; CELLS |
| 收录类别 | SCI |
| 语种 | 中文 |
| WOS记录号 | WOS:000337885500005 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/203]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Li, HY,Zhou, ZM,Pan, Q,et al. A Study of Three Linker Peptides' Cleavage Efficiency at The Post-translational Level[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2014,41(6):558-566. |
| APA | Li, HY.,Zhou, ZM.,Pan, Q.,Yang, DM.,Gu, JF.,...&Zhang, KJ.(2014).A Study of Three Linker Peptides' Cleavage Efficiency at The Post-translational Level.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,41(6),558-566. |
| MLA | Li, HY,et al."A Study of Three Linker Peptides' Cleavage Efficiency at The Post-translational Level".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 41.6(2014):558-566. |
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