Generation of neural progenitor cells by chemical cocktails and hypoxia
文献类型：期刊论文
| 作者 | Cheng, L; Hu, WX; Qiu, BL; Zhao, J; Yu, YC; Guan, WQ; Wang, M; Yang, WZ; Pei, G |
| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2014 |
| 卷号 | 24期号:6页码:665-679 |
| 关键词 | lineage conversion neural progenitor cells chemical cocktails hypoxia |
| 通讯作者 | Pei, G (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Cell Biol, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,jzhao@sibs.ac.cn ; gpei@sibs.ac.cn |
| 英文摘要 | Neural progenitor cells (NPCs) can be induced from somatic cells by defined factors. Here we report that NPCs can be generated from mouse embryonic fibroblasts by a chemical cocktail, namely VCR (V, VPA, an inhibitor of HDACs; C, CHIR99021, an inhibitor of GSK-3 kinases and R, Repsox, an inhibitor of TGF-beta pathways), under a physiological hypoxic condition. These chemical-induced NPCs (ciNPCs) resemble mouse brain-derived NPCs regarding their proliferative and self-renewing abilities, gene expression profiles, and multipotency for different neuroectodermal lineages in vitro and in vivo. Further experiments reveal that alternative cocktails with inhibitors of histone deacetylation, glycogen synthase kinase, and TGF-beta pathways show similar efficacies for ciNPC induction. Moreover, ciNPCs can also be induced from mouse tail-tip fibroblasts and human urinary cells with the same chemical cocktail VCR. Thus our study demonstrates that lineage-specific conversion of somatic cells to NPCs could be achieved by chemical cocktails without introducing exogenous factors. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | PLURIPOTENT STEM-CELLS ; SMALL-MOLECULE COMPOUNDS ; CENTRAL-NERVOUS-SYSTEM ; DEFINED FACTORS ; MOUSE FIBROBLASTS ; DIRECT CONVERSION ; SOMATIC-CELLS ; BRAIN ; ACTIVATION ; GENES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000337682900007 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/204]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Cheng, L,Hu, WX,Qiu, BL,et al. Generation of neural progenitor cells by chemical cocktails and hypoxia[J]. CELL RESEARCH,2014,24(6):665-679. |
| APA | Cheng, L.,Hu, WX.,Qiu, BL.,Zhao, J.,Yu, YC.,...&Pei, G.(2014).Generation of neural progenitor cells by chemical cocktails and hypoxia.CELL RESEARCH,24(6),665-679. |
| MLA | Cheng, L,et al."Generation of neural progenitor cells by chemical cocktails and hypoxia".CELL RESEARCH 24.6(2014):665-679. |
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