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Pachytene piRNAs instruct massive mRNA elimination during late spermiogenesis
文献类型：期刊论文
| 作者 | Gou, LT; Dai, P; Yang, JH; Xue, YC; Hu, YP; Zhou, Y; Kang, JY; Wang, X; Li, HR; Hua, MM |
| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2014 |
| 卷号 | 24期号:6页码:680-700 |
| 关键词 | pachytene piRNAs MIWI CAF1 pi-RISC mRNA deadenylation ES |
| 通讯作者 | Liu, MF (reprint author), Univ Chinese Acad Sci, Ctr RNA Res, State Key Lab Mol Biol, Shanghai 200031, Peoples R China.,mfliu@sibcb.ac.cn |
| 英文摘要 | Spermatogenesis in mammals is characterized by two waves of piRNA expression: one corresponds to classic piRNAs responsible for silencing retrotransponsons and the second wave is predominantly derived from nontransposon intergenic regions in pachytene spermatocytes, but the function of these pachytene piRNAs is largely unknown. Here, we report the involvement of pachytene piRNAs in instructing massive mRNA elimination in mouse elongating spermatids (ES). We demonstrate that a piRNA-induced silencing complex (pi-RISC) containing murine PIWI (MIWI) and deadenylase CAF1 is selectively assembled in ES, which is responsible for inducing mRNA deadenylation and decay via a mechanism that resembles the action of miRNAs in somatic cells. Such a highly orchestrated program appears to take full advantage of the enormous repertoire of diversified targeting capacity of pachytene piRNAs derived from nontransposon intergenic regions. These findings suggest that pachytene piRNAs are responsible for inactivating vast cellular programs in preparation for sperm production from ES. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | NOVO DNA METHYLATION ; MALE GERM-CELLS ; FUNCTIONAL INSIGHTS ; ELEGANS PIRNAS ; PIWI PROTEINS ; SPERMATOGENESIS ; MOUSE ; MIWI ; DROSOPHILA ; COMPLEXES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000337682900008 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/205]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Gou, LT,Dai, P,Yang, JH,et al. Pachytene piRNAs instruct massive mRNA elimination during late spermiogenesis[J]. CELL RESEARCH,2014,24(6):680-700. |
| APA | Gou, LT.,Dai, P.,Yang, JH.,Xue, YC.,Hu, YP.,...&Liu, MF.(2014).Pachytene piRNAs instruct massive mRNA elimination during late spermiogenesis.CELL RESEARCH,24(6),680-700. |
| MLA | Gou, LT,et al."Pachytene piRNAs instruct massive mRNA elimination during late spermiogenesis".CELL RESEARCH 24.6(2014):680-700. |
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