Structural basis for protein-RNA recognition in telomerase
文献类型：期刊论文
| 作者 | Huang, J; Brown, AF; Wu, J; Xue, J; Bley, CJ; Rand, DP; Wu, LJ; Zhang, RG; Chen, JJL; Lei, M |
| 刊名 | NATURE STRUCTURAL & MOLECULAR BIOLOGY
 |
| 出版日期 | 2014 |
| 卷号 | 21期号:6页码:507-512 |
| 通讯作者 | Lei, M (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Natl Ctr Prot Sci Shanghai,State Key Lab Mol Biol, Shanghai, Peoples R China.,jlchen@asu.edu ; leim@sibcb.ac.cn |
| 英文摘要 | Telomerase is a large ribonucleoprotein complex minimally composed of a catalytic telomerase reverse transcriptase (TERT) and an RNA component (TR) that provides the template for telomeric DNA synthesis. However, it remains unclear how TERT and TR assemble into a functional telomerase. Here we report the crystal structure of the conserved regions 4 and 5 (CR4/5) of TR in complex with the TR-binding domain (TRBD) of TERT from the teleost fish Oryzias latipes. The structure shows that CR4/5 adopts an L-shaped three-way-junction conformation with its two arms clamping onto TRBD. Both the sequence and conformation of CR4/5 are required for the interaction. Our structural and mutational analyses suggest that the observed CR4/5-TRBD recognition is common to most eukaryotes, and CR4/5 in vertebrate TR might have a similar role in telomerase regulation as that of stem-loop IV in Tetrahymena TR. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics; Cell Biology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics ; Cell Biology |
| 关键词[WOS] | CATALYTIC SUBUNIT TERT ; STEM-LOOP IV ; DYSKERATOSIS-CONGENITA ; REVERSE-TRANSCRIPTASE ; TRIPLE-HELIX ; DOMAIN ; BINDING ; CANCER ; THERAPEUTICS ; PROCESSIVITY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000337010500004 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/206]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Huang, J,Brown, AF,Wu, J,et al. Structural basis for protein-RNA recognition in telomerase[J]. NATURE STRUCTURAL & MOLECULAR BIOLOGY,2014,21(6):507-512. |
| APA | Huang, J.,Brown, AF.,Wu, J.,Xue, J.,Bley, CJ.,...&Lei, M.(2014).Structural basis for protein-RNA recognition in telomerase.NATURE STRUCTURAL & MOLECULAR BIOLOGY,21(6),507-512. |
| MLA | Huang, J,et al."Structural basis for protein-RNA recognition in telomerase".NATURE STRUCTURAL & MOLECULAR BIOLOGY 21.6(2014):507-512. |
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