Regulation of Arabidopsis Flowering by the Histone Mark Readers MRG1/2 via Interaction with CONSTANS to Modulate FT Expression
文献类型：期刊论文
| 作者 | Bu, ZY; Yu, Y; Li, ZP; Liu, YC; Jiang, W; Huang, Y; Dong, AW |
| 刊名 | PLOS GENETICS
 |
| 出版日期 | 2014 |
| 卷号 | 10期号:9页码:e1004617-e1004617 |
| 通讯作者 | Bu, ZY (reprint author), Fudan Univ, Sch Life Sci, Inst Plant Biol, State Key Lab Genet Engn,Collaborat Innovat Ctr G, Shanghai 200433, Peoples R China.,aiwudong@fudan.edu.cn |
| 英文摘要 | Day-length is important regulating the transition to reproductive development (flowering) in plants. In the de plan Arabidopsis thaliana, the transcription factor CONSTANS (CO) promotes expression of the florigen FLOWERING LOCUS T (F constituting a key flowering pathway under long-day photoperiods. Recent studies have revealed that FT expression is regulated by changes of histone modification marks of the FT chromatin, but the epigenetic regulators that directly interact with the CO protein have not been identified. Here, we show that the Arabidopsis Morf Related Gene (MI3G) group proteins MRG1 and MRG2 act as H3K4me3/H3K36me3 readers and physically interact with CO to activate FT expression. In vitro binding analyses indicated that the chromodomains of MRG1 and MRG2 preferentially bind H3K4me3/H3K36me3 peptides. The mrg1 mrg2 double mutant exhibits reduced mRNA levels of FT, but not of CO, and shows a late-flowering phenotype under the long-day but not short-day photoperiod growth conditions. MRG2 associates with the chromatin of FT promoter in a ay dependent of both CO and H3K4me3/H3K36me3. Vice versa, loss I of MRG1 and MRG2 also impairs CO binding at the is FT promoter. Crystal structure analyses of MRG2 bound with H3K4me3/H3K36me3 peptides together with mutagenesis analysis in p anta further demonstrated that MRG2 function relies on its H3K4me3/H3K36me3-binding activity. Collectively, our results unravel a novel chromatin regulatory mechanism, linking functions of MRG1 and MRG2 protein H3K4/H3K36 methylations, and CO in FT activation in the photoperiodic regulation of flowering time in plants. |
| 学科主题 | Genetics & Heredity |
| 类目[WOS] | Genetics & Heredity |
| 关键词[WOS] | H3 LYSINE 27 ; LOCUS-T ; TRANSCRIPTIONAL ACTIVATION ; SACCHAROMYCES-CEREVISIAE ; CHROMO DOMAIN ; CW DOMAIN ; PROTEIN ; TRIMETHYLATION ; METHYLATION ; REPRESSION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000343009600037 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/218]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Bu, ZY,Yu, Y,Li, ZP,et al. Regulation of Arabidopsis Flowering by the Histone Mark Readers MRG1/2 via Interaction with CONSTANS to Modulate FT Expression[J]. PLOS GENETICS,2014,10(9):e1004617-e1004617. |
| APA | Bu, ZY.,Yu, Y.,Li, ZP.,Liu, YC.,Jiang, W.,...&Dong, AW.(2014).Regulation of Arabidopsis Flowering by the Histone Mark Readers MRG1/2 via Interaction with CONSTANS to Modulate FT Expression.PLOS GENETICS,10(9),e1004617-e1004617. |
| MLA | Bu, ZY,et al."Regulation of Arabidopsis Flowering by the Histone Mark Readers MRG1/2 via Interaction with CONSTANS to Modulate FT Expression".PLOS GENETICS 10.9(2014):e1004617-e1004617. |
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