A Ubiquitin Shuttle DC-UbP/UBTD2 Reconciles Protein Ubiquitination and Deubiquitination via Linking UbE1 and USP5 Enzymes
文献类型：期刊论文
| 作者 | Song, AX; Yang, H; Gao, YG; Zhou, CJ; Zhang, YH; Hu, HY |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2014 |
| 卷号 | 9期号:9页码:e107509-e107509 |
| 通讯作者 | Hu, HY (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai, Peoples R China.,hyhu@sibcb.ac.cn |
| 英文摘要 | The ubiquitination levels of protein substrates in eukaryotic cells are delicately orchestrated by various protein cofactors and enzymes. Dendritic cell-derived ubiquitin (Ub)-like protein (DC-UbP), also named as Ub domain-containing protein 2 (UBTD2), is a potential Ub shuttle protein comprised of a Ub-like (UbL) domain and a Ub-binding domain (UBD), but its biological function remains largely unknown. We identified two Ub-related enzymes, the deubiquitinating enzyme USP5 and the Ub-activating enzyme UbE1, as interacting partners of DC-UbP from HEK 293T cells. Biochemical studies revealed that the tandem UBA domains of USP5 and the C-terminal Ub-fold domain (UFD) of UbE1 directly interacted with the C-terminal UbL domain of DC-UbP but on the distinct surfaces. Overexpression of DC-UbP in HEK 293T cells enhanced the association of these two enzymes and thus prompted cellular ubiquitination, whereas knockdown of the protein reduced the cellular ubiquitination level. Together, DC-UbP may integrate the functions of USP5 and UbE1 through interacting with them, and thus reconcile the cellular ubiquitination and deubiquitination processes. |
| 学科主题 | Science & Technology - Other Topics |
| 类目[WOS] | Multidisciplinary Sciences |
| 关键词[WOS] | ACTIVATING ENZYME ; POLYUBIQUITIN CHAINS ; DENDRITIC CELLS ; ISOPEPTIDASE-T ; DC-UBP ; BINDING ; DOMAIN ; DEGRADATION ; LIGASE ; E1 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000342030300088 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/220]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Song, AX,Yang, H,Gao, YG,et al. A Ubiquitin Shuttle DC-UbP/UBTD2 Reconciles Protein Ubiquitination and Deubiquitination via Linking UbE1 and USP5 Enzymes[J]. PLOS ONE,2014,9(9):e107509-e107509. |
| APA | Song, AX,Yang, H,Gao, YG,Zhou, CJ,Zhang, YH,&Hu, HY.(2014).A Ubiquitin Shuttle DC-UbP/UBTD2 Reconciles Protein Ubiquitination and Deubiquitination via Linking UbE1 and USP5 Enzymes.PLOS ONE,9(9),e107509-e107509. |
| MLA | Song, AX,et al."A Ubiquitin Shuttle DC-UbP/UBTD2 Reconciles Protein Ubiquitination and Deubiquitination via Linking UbE1 and USP5 Enzymes".PLOS ONE 9.9(2014):e107509-e107509. |
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