Parallel detection and quantitative analysis of specific binding of proteins by oblique-incidence reflectivity difference technique in label-free format
文献类型：期刊论文
| 作者 | Dai, J; Li, L; He, LP; Ruan, KC; Lu, HB; Jin, KJ; Yang, GZ |
| 刊名 | SCIENCE CHINA-PHYSICS MECHANICS & ASTRONOMY
 |
| 出版日期 | 2014 |
| 卷号 | 57期号:11页码:2039-2042 |
| 关键词 | oblique-incidence reflectivity difference (OIRD) protein microarray label-free detection quantitative analysis |
| 通讯作者 | Lu, HB (reprint author), Chinese Acad Sci, Beijing Natl Lab Condensed Matter Phys, Inst Phys, Beijing 100190, Peoples R China.,hblu@iphy.ac.cn |
| 英文摘要 | In this work, we parallelly detected the specific binding between microarray targets including 12 different kinds of proteins and the probe solution containing five corresponding antibodies and quantitatively analyzed the interactions between CDH13 and solution phase anti-CDH13 at six different probe concentrations by oblique-incidence reflectivity difference (OIRD) method in label-free format. The detection sensitivity reached 10 ng/mL. The experimental results indicate that the OIRD method is a promising and competing technique not only in research work but also in clinic. |
| 学科主题 | Physics |
| 类目[WOS] | Physics, Multidisciplinary |
| 关键词[WOS] | REAL-TIME DETECTION ; MICROARRAYS ; PROSPECTS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000343191600004 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/242]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Dai, J,Li, L,He, LP,et al. Parallel detection and quantitative analysis of specific binding of proteins by oblique-incidence reflectivity difference technique in label-free format[J]. SCIENCE CHINA-PHYSICS MECHANICS & ASTRONOMY,2014,57(11):2039-2042. |
| APA | Dai, J.,Li, L.,He, LP.,Ruan, KC.,Lu, HB.,...&Yang, GZ.(2014).Parallel detection and quantitative analysis of specific binding of proteins by oblique-incidence reflectivity difference technique in label-free format.SCIENCE CHINA-PHYSICS MECHANICS & ASTRONOMY,57(11),2039-2042. |
| MLA | Dai, J,et al."Parallel detection and quantitative analysis of specific binding of proteins by oblique-incidence reflectivity difference technique in label-free format".SCIENCE CHINA-PHYSICS MECHANICS & ASTRONOMY 57.11(2014):2039-2042. |
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