Phosphorylation of cyclin Y by CDK14 induces its ubiquitination and degradation
文献类型：期刊论文
| 作者 | Li, S; Song, W; Jiang, M; Zeng, LY; Zhu, XL; Chen, JY |
| 刊名 | FEBS LETTERS
 |
| 出版日期 | 2014 |
| 卷号 | 588期号:11页码:1989-1996 |
| 关键词 | Cyclin Y CDK14 Phosphorylation Ubiquitination Protein turnover |
| 通讯作者 | Chen, JY (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,jychen@sibcb.ac.cn |
| 英文摘要 | Cyclin Y, a membrane associated cyclin, is capable of binding and activating CDK14. Here we report that human cyclin Y (CCNY) is a phosphoprotein in vivo and that phosphorylation of CCNY by CDK14 triggers its ubiquitination and degradation. Inactivation of either CDK14 or Cul1 results in accumulation of CCNY. An in vivo and in vitro mapping of CCNY phosphorylation sites by mass spectrometry revealed that the flanking regions of the conserved cyclin box are heavily phosphorylated. Phosphorylation of CCNY at Serines 71 and 73 creates a putative phospho-degron that controls its association with an SCF complex. Mutation of serine to alanine at these two sites stabilized CCNY and enhanced the activity of CCNY/CDK14 on phosphorylation of LRP6. Our results provide insight into autoregulation of the cyclin Y/CDK14 pair in CDK14 activation and cyclin Y turnover which is a process that is involved in membrane proximal signaling. (c) 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics; Cell Biology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics ; Cell Biology |
| 关键词[WOS] | CELL-CYCLE ; DEPENDENT UBIQUITINATION ; PROTEASOME PATHWAY ; CONSERVED CYCLIN ; LIGASE COMPLEX ; PROTEIN ; KINASE ; IDENTIFICATION ; INHIBITION ; ACTIVATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000336394100001 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/243]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Li, S,Song, W,Jiang, M,et al. Phosphorylation of cyclin Y by CDK14 induces its ubiquitination and degradation[J]. FEBS LETTERS,2014,588(11):1989-1996. |
| APA | Li, S,Song, W,Jiang, M,Zeng, LY,Zhu, XL,&Chen, JY.(2014).Phosphorylation of cyclin Y by CDK14 induces its ubiquitination and degradation.FEBS LETTERS,588(11),1989-1996. |
| MLA | Li, S,et al."Phosphorylation of cyclin Y by CDK14 induces its ubiquitination and degradation".FEBS LETTERS 588.11(2014):1989-1996. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。