Osteogenic Differentiation of Human Amniotic Epithelial Cells and Its Application in Alveolar Defect Restoration
文献类型：期刊论文
| 作者 | Si, JW; Zhang, JJ; Dai, JW; Yu, DD; Yu, HB; Shi, J; Wang, XD; Shen, SGF; Guo, LH |
| 刊名 | STEM CELLS TRANSLATIONAL MEDICINE
 |
| 出版日期 | 2014 |
| 卷号 | 3期号:12页码:1504-1513 |
| 关键词 | Human amniotic epithelial cells Osteogenesis Alveolar defect Host immune response |
| 通讯作者 | Shen, SGF (reprint author), Shanghai Jiao Tong Univ, Peoples Hosp 9, Coll Stomatol, Dept Oral & Craniomaxillofacial Sci,Shanghai Key, 639 Zhizaoju Rd, Shanghai 200011, Peoples R China.,maxillofacsurg@163.com ; guolihe@cell-star.com.cn |
| 英文摘要 | The present study investigated the detailed in vitro osteogenic differentiation process and in vivo bone regenerative property of human amniotic epithelial cells (hAECs). The in vitro osteogenic differentiation process of hAECs was evaluated by biochemical staining, real-time polymerase chain reaction, and immunofluorescence. Next, beta-tricalcium phosphate (beta-TCP) scaffolds alone or loaded with hAECs were implanted into the alveolar defects of rats. Micro-computed tomography evaluation and histologic studies were conducted. Our results validated the in vitro osteogenic capacity of hAECs by upregulation of Runx2, osterix, alkaline phosphatase, collagen I, and osteopontin, with positive biochemical staining for osteoblasts. An epithelial-mesenchymal transformation process might be involved in the osteogenic differentiation of hAECs by increased expression of transforming growth factor-beta 1. Our data also demonstrated that in vivo implantation of hArCs loaded on beta-TCP scaffolds, not only improved bone regeneration by direct participation, but also reduced the early host immune response to the scaffolds. The presented data indicate that hAECs possess proper osteogenic differentiation potential and a modulatory influence on the early tissue remodeling process, making these cells a potential source of progenitor cells for clinical restoration of the alveolar defect. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell & Tissue Engineering |
| 关键词[WOS] | MESENCHYMAL STEM-CELLS ; BONE TISSUE ; ADIPOSE-TISSUE ; CLEFT DEFECT ; REPAIR ; REGENERATION ; TRANSITION ; MODEL |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000346460900027 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/247]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Si, JW,Zhang, JJ,Dai, JW,et al. Osteogenic Differentiation of Human Amniotic Epithelial Cells and Its Application in Alveolar Defect Restoration[J]. STEM CELLS TRANSLATIONAL MEDICINE,2014,3(12):1504-1513. |
| APA | Si, JW.,Zhang, JJ.,Dai, JW.,Yu, DD.,Yu, HB.,...&Guo, LH.(2014).Osteogenic Differentiation of Human Amniotic Epithelial Cells and Its Application in Alveolar Defect Restoration.STEM CELLS TRANSLATIONAL MEDICINE,3(12),1504-1513. |
| MLA | Si, JW,et al."Osteogenic Differentiation of Human Amniotic Epithelial Cells and Its Application in Alveolar Defect Restoration".STEM CELLS TRANSLATIONAL MEDICINE 3.12(2014):1504-1513. |
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