GFP Stable Transfection Facilitated the Characterization of Lung Cancer Stem Cells
文献类型：期刊论文
| 作者 | Li, N; Yang, Y; Ding, M; Huang, WD; Li, HG; Ye, J; Xiao, J; Zha, XL; Xu, HN |
| 刊名 | MOLECULAR BIOTECHNOLOGY
 |
| 出版日期 | 2014 |
| 卷号 | 56期号:12页码:1079-1088 |
| 关键词 | Lung cancer stem cell Flow cytometry Chemoresistance Quiescence Differentiation |
| 通讯作者 | Zha, XL (reprint author), Fudan Univ, Dept Biochem & Mol Biol, Shanghai Med Coll, 138 Yi Xue Yuan Rd, Shanghai 200032, Peoples R China.,xlzha@shmu.edu.cn ; xuhaineng123@163.com |
| 英文摘要 | Cancer stem cells (CSCs) are a subset of cancer cells that play key roles in metastasis and cancer relapse. The elimination of CSCs is very important during cancer therapy. To develop drugs that target CSCs, the isolation and identification of putative CSCs are required. Some of the characteristics of CSCs are assessed by cell survival assays. In such experiments, the density of the cells seeded on the plates may affect the experimental results, leading to potentially inaccurate conclusions. In this study, a new assay to facilitate the characterization of CSCs has been developed by stable transfection of GFP, using the A549 lung cancer cell line as a model. A putative CSC line, A549 sphere cells, was obtained by culturing A549 cells in ultra-low dishes in serum-free medium. To ensure that the putative CSCs were grown under the same conditions as the A549-GFP cells and were not affected by the number of cells seeded, A549 sphere cells were mixed with GFP stably transfected A549 (A549-GFP) cells. The mixture was subjected to flow cytometry assay and inverted fluorescence microscopy to detect changes in the proportion of GFP-positive cells after treatment. A549 sphere cells had a slower proliferation rate and an improved chemoresistance. They also showed differentiation ability. This work suggests that mixing GFP stably transfected cancer cells with putative CSCs may facilitate the identification of CSCs, making it convenient for studies of targeted CSCs. |
| 学科主题 | Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 关键词[WOS] | TUMOR-INITIATING CELLS ; HEPATOCELLULAR-CARCINOMA CELLS ; LYMPHOCYTIC-LEUKEMIA CELLS ; LIVER-CANCER ; IDENTIFICATION ; PATHWAY ; CD133 ; POPULATION ; INHIBITION ; HEDGEHOG |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000344484700002 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/252]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Li, N,Yang, Y,Ding, M,et al. GFP Stable Transfection Facilitated the Characterization of Lung Cancer Stem Cells[J]. MOLECULAR BIOTECHNOLOGY,2014,56(12):1079-1088. |
| APA | Li, N.,Yang, Y.,Ding, M.,Huang, WD.,Li, HG.,...&Xu, HN.(2014).GFP Stable Transfection Facilitated the Characterization of Lung Cancer Stem Cells.MOLECULAR BIOTECHNOLOGY,56(12),1079-1088. |
| MLA | Li, N,et al."GFP Stable Transfection Facilitated the Characterization of Lung Cancer Stem Cells".MOLECULAR BIOTECHNOLOGY 56.12(2014):1079-1088. |
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