Sumoylation of Influenza A Virus Nucleoprotein Is Essential for Intracellular Trafficking and Virus Growth
文献类型：期刊论文
| 作者 | Han, QL; Chang, C; Li, L; Klenk, C; Cheng, JK; Chen, YX; Xia, NS; Shu, YL; Chen, Z; Gabriel, G |
| 刊名 | JOURNAL OF VIROLOGY
 |
| 出版日期 | 2014 |
| 卷号 | 88期号:16页码:9379-9390 |
| 通讯作者 | Xu, K (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Pasteur Shanghai, Mol Virol Unit,Key Lab Mol Virol & Immunol, Shanghai, Peoples R China.,bsun@sibs.ac.cn ; kxu@sibs.ac.cn |
| 英文摘要 | Viruses take advantage of host posttranslational modifications for their own benefit. It was recently reported that influenza A virus proteins interact extensively with the host sumoylation system. Thereby, several viral proteins, including NS1 and M1, are sumoylated to facilitate viral replication. However, to what extent sumoylation is exploited by influenza A virus is not fully understood. In this study, we found that influenza A virus nucleoprotein (NP) is a bona fide target of sumoylation in both NP-transfected cells and virus-infected cells. We further found that NP is sumoylated at the two most N-terminal residues, lysines 4 and 7, and that sumoylation at lysine 7 of NP is highly conserved across different influenza A virus subtypes and strains, including the recently emerged human H7N9 virus. While NP stability and polymerase activity are little affected by sumoylation, the NP sumoylation-defective WSN-NPK4,7R virus exhibited early cytoplasmic localization of NP. The growth of the WSN-NPK4,7R virus was highly attenuated compared to that of the wild-type WSN virus, and the lysine residue at position 7 is indispensable for the virus's survival, as illustrated by the rapid emergence of revertant viruses. Thus, sumoylation of influenza A virus NP is essential for intracellular trafficking of NP and for virus growth, illustrating sumoylation as a crucial strategy extensively exploited by influenza A virus for survival in its host. |
| 学科主题 | Virology |
| 类目[WOS] | Virology |
| 关键词[WOS] | INFECTED-CELLS ; PROTEIN ; SUMO ; TRANSCRIPTION ; REPLICATION ; STABILITY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000340036800054 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/257]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Han, QL,Chang, C,Li, L,et al. Sumoylation of Influenza A Virus Nucleoprotein Is Essential for Intracellular Trafficking and Virus Growth[J]. JOURNAL OF VIROLOGY,2014,88(16):9379-9390. |
| APA | Han, QL.,Chang, C.,Li, L.,Klenk, C.,Cheng, JK.,...&Xu, K.(2014).Sumoylation of Influenza A Virus Nucleoprotein Is Essential for Intracellular Trafficking and Virus Growth.JOURNAL OF VIROLOGY,88(16),9379-9390. |
| MLA | Han, QL,et al."Sumoylation of Influenza A Virus Nucleoprotein Is Essential for Intracellular Trafficking and Virus Growth".JOURNAL OF VIROLOGY 88.16(2014):9379-9390. |
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