The mRNA of Human Cytoplasmic Arginyl-tRNA Synthetase Recruits Prokaryotic Ribosomes Independently
文献类型：期刊论文
| 作者 | Yang, F; Ji, QQ; Ruan, LL; Ye, Q; Wang, ED |
| 刊名 | JOURNAL OF BIOLOGICAL CHEMISTRY
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| 出版日期 | 2014 |
| 卷号 | 289期号:30页码:20953-20959 |
| 通讯作者 | Wang, ED (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,edwang@sibcb.ac.cn |
| 英文摘要 | There are two isoforms of cytoplasmic arginyl-tRNA synthetase (hcArgRS) in human cells. The long form is a component of the multiple aminoacyl-tRNA synthetase complex, and the other is an N-terminal truncated form (Delta NhcArgRS), free in the cytoplasm. It has been shown that the two forms of ArgRS arise from alternative translational initiation in a single mRNA. The short form is produced from the initiation at a downstream, in-frame AUG start codon. Interestingly, our data suggest that the alternative translational initiation of hcArgRS mRNA also takes place in Escherichia coli transformants. When the gene encoding full-length hcArgRS was overexpressed in E. coli, two forms of hcArgRS were observed. The N-terminal sequencing experiment identified that the short form was identical to the Delta NhcArgRS in human cytoplasm. By constructing a bicistronic system, our data support that the mRNA encoding the N-terminal extension of hcArgRS has the capacity of independently recruiting E. coli ribosomes. Furthermore, two critical elements for recruiting prokaryotic ribosomes were identified, the "AGGA" core of the Shine-Dalgarno sequence and the "A-rich" sequence located just proximal to the alternative in-frame initiation site. Although the mechanisms of prokaryotic and eukaryotic translational initiation are distinct, they share some common features. The ability of the hcArgRS mRNA to recruit the prokaryotic ribosome may provide clues for shedding light on the mechanism of alternative translational initiation of hcArgRS mRNA in eukaryotic cells. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | AMINOACYL-TRANSFER RNA ; TRANSLATION INITIATION ; SECONDARY STRUCTURE ; 2 FORMS ; REINITIATION ; SEQUENCE ; EUKARYOTES ; COMPLEX ; DOMAIN ; SHINE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000339396600048 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/267]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Yang, F,Ji, QQ,Ruan, LL,et al. The mRNA of Human Cytoplasmic Arginyl-tRNA Synthetase Recruits Prokaryotic Ribosomes Independently[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2014,289(30):20953-20959. |
| APA | Yang, F,Ji, QQ,Ruan, LL,Ye, Q,&Wang, ED.(2014).The mRNA of Human Cytoplasmic Arginyl-tRNA Synthetase Recruits Prokaryotic Ribosomes Independently.JOURNAL OF BIOLOGICAL CHEMISTRY,289(30),20953-20959. |
| MLA | Yang, F,et al."The mRNA of Human Cytoplasmic Arginyl-tRNA Synthetase Recruits Prokaryotic Ribosomes Independently".JOURNAL OF BIOLOGICAL CHEMISTRY 289.30(2014):20953-20959. |
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