Acetylcholinesterase overexpression mediated by oncolytic adenovirus exhibited potent anti-tumor effect
文献类型：期刊论文
| 作者 | Xu, HN; Shen, ZX; Xiao, J; Yang, Y; Huang, WD; Zhou, ZM; Shen, JN; Zhu, YH; Liu, XY; Chu, L |
| 刊名 | BMC CANCER
 |
| 出版日期 | 2014 |
| 卷号 | 14期号:1页码:668-668 |
| 关键词 | AChE Oncolytic adenovirus Gastric cancer Apoptosis |
| 通讯作者 | Zhu, YH (reprint author), Fudan Univ, Sch Pharm, Dept Pharmacol, Shanghai 201203, Peoples R China.,zhuyz@fudan.edu.cn ; xyliu@sibcb.ac.cn ; lchu@sibcb.ac.cn |
| 英文摘要 | Background: Acetylcholinesterase (AChE) mainly functions as an efficient terminator for acetylcholine signaling transmission. Here, we reported the effect of AChE on gastric cancer therapy. Methods: The expression of AChE in gastric cancerous tissues and adjacent non-cancerous tissues was examined by immunohistochemistry. Gastric cancer cells were treated with AChE delivered by replication-deficient adenoviral vector (Ad. AChE) or oncolytic adenoviral vector (ZD55-AChE), respectively, followed by measurement of cell viability and apoptosis by MTT assay and apoptosis detection assays. In vivo, the tumor growth of gastric cancer xenografts in mice treated with Ad. AChE or ZD55-AChE (1 x 10(9) PFU) were measured. In addition, the cell viability of gastric cancer stem cells treated with Ad. AChE or ZD55-AChE were evaluated by MTT assay. Results: A positive correlation was found between higher level of AChE expression in gastric cancer patient samples and longer survival time of the patients. Ad. AChE and ZD55-AChE inhibited gastric cancer cell growth, and low dose of ZD55-AChE induced mitochondrial pathway of apoptosis in cells. ZD55-AChE repressed tumor growth in vivo, and the anti-tumor efficacy is greater than Ad. AChE. Moreover, ZD55-AChE suppressed the growth of gastric cancer stem cells. Conclusion: ZD55-AChE represented potential therapeutic effect for human gastric cancer. |
| 学科主题 | Oncology |
| 类目[WOS] | Oncology |
| 关键词[WOS] | CANCER STEM-CELLS ; CHOLINESTERASE ACTIVITY ; GENE NANOCOMPLEX ; TARGETED THERAPY ; GASTRIC-CANCER ; ACHE-R ; CARCINOMA ; PROLIFERATION ; EXPRESSION ; APOPTOSIS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000341658600001 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/282]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Xu, HN,Shen, ZX,Xiao, J,et al. Acetylcholinesterase overexpression mediated by oncolytic adenovirus exhibited potent anti-tumor effect[J]. BMC CANCER,2014,14(1):668-668. |
| APA | Xu, HN.,Shen, ZX.,Xiao, J.,Yang, Y.,Huang, WD.,...&Chu, L.(2014).Acetylcholinesterase overexpression mediated by oncolytic adenovirus exhibited potent anti-tumor effect.BMC CANCER,14(1),668-668. |
| MLA | Xu, HN,et al."Acetylcholinesterase overexpression mediated by oncolytic adenovirus exhibited potent anti-tumor effect".BMC CANCER 14.1(2014):668-668. |
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