O18Quant: A Semiautomatic Strategy for Quantitative Analysis of High-Resolution O-16/O-18 Labeled Data
文献类型：期刊论文
| 作者 | Guo, Y; Miyagi, M; Zeng, R; Sheng, QH |
| 刊名 | BIOMED RESEARCH INTERNATIONAL
 |
| 出版日期 | 2014 |
| 期号 | 1页码:971857-971857 |
| 通讯作者 | Sheng, QH (reprint author), Vanderbilt Univ, Ctr Quantitat Sci, Nashville, TN 37027 USA.,qhsheng@sibs.ac.cn |
| 英文摘要 | Proteolytic O-18-labeling has been widely used in quantitative proteomics since it can uniformly label all peptides from different kinds of proteins. There have been multiple algorithms and tools developed over the last few years to analyze high-resolution proteolytic O-16/O-18 labeled mass spectra. We have developed a software package, O18Quant, which addresses two major issues in the previously developed algorithms. First, O18Quant uses a robust linear model (RLM) for peptide-to-protein ratio estimation. RLM can minimize the effect of outliers instead of iteratively removing them which is a common practice in other approaches. Second, the existing algorithms lack applicable implementation. We address this by implementing O18Quant using C# under Microsoft.net framework and R. O18Quant automatically calculates the peptide/protein relative ratio and provides a friendly graphical user interface (GUI) which allows the user to manually validate the quantification results at scan, peptide, and protein levels. The intuitive GUI of O18Quant can greatly enhance the user's visualization and understanding of the data analysis. O18Quant can be downloaded for free as part of the software suite Proteomics Tools. |
| 学科主题 | Biotechnology & Applied Microbiology; Research & Experimental Medicine |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Medicine, Research & Experimental |
| 关键词[WOS] | MASS-SPECTROMETRY ; PROTEOMICS ; PEPTIDES ; PROTEIN ; ABUNDANCE ; EXCHANGE ; YEAST ; TOOL |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000336304500001 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/291]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Guo, Y,Miyagi, M,Zeng, R,et al. O18Quant: A Semiautomatic Strategy for Quantitative Analysis of High-Resolution O-16/O-18 Labeled Data[J]. BIOMED RESEARCH INTERNATIONAL,2014(1):971857-971857. |
| APA | Guo, Y,Miyagi, M,Zeng, R,&Sheng, QH.(2014).O18Quant: A Semiautomatic Strategy for Quantitative Analysis of High-Resolution O-16/O-18 Labeled Data.BIOMED RESEARCH INTERNATIONAL(1),971857-971857. |
| MLA | Guo, Y,et al."O18Quant: A Semiautomatic Strategy for Quantitative Analysis of High-Resolution O-16/O-18 Labeled Data".BIOMED RESEARCH INTERNATIONAL .1(2014):971857-971857. |
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