Complementation of Hyponastic Leaves1 by Double-Strand RNA-Binding Domains of Dicer-Like1 in Nuclear Dicing Bodies
文献类型：期刊论文
| 作者 | Liu, Q; Yan, QQ; Liu, Y; Hong, F; Sun, ZF; Shi, LL; Huang, Y; Fang, YD |
| 刊名 | PLANT PHYSIOLOGY
 |
| 出版日期 | 2013 |
| 卷号 | 163期号:1页码:108-117 |
| 通讯作者 | Fang, YD (reprint author), Chinese Acad Sci, Shanghai Inst Plant Physiol & Ecol, Natl Key Lab Plant Mol Genet, Shanghai Inst Biol Sci, Shanghai 200032, Peoples R China.,yfang@sippe.ac.cn |
| 英文摘要 | MicroRNAs (miRNAs) are a class of small regulatory RNAs that are found in almost all of the eukaryotes. Arabidopsis (Arabidopsis thaliana) miRNAs are processed from primary miRNAs (pri-miRNAs), mainly by the ribonuclease III-like enzyme DICER-LIKE1 (DCL1) and its specific partner, HYPONASTIC LEAVES1 (HYL1), a double-strand RNA-binding protein, both of which contain two double-strand RNA-binding domains (dsRBDs). These dsRBDs are essential for miRNA processing, but the functions of them are not clear. Here, we report that the two dsRBDs of DCL1 (DCL1-D1D2), and to some extent the second dsRBD (DCL1-D2), complement the hyl1 mutant, but not the first dsRBD of DCL1 (DCL1-D1). DCL1-D1 is diffusely distributed throughout the nucleoplasm, whereas DCL1-D2 and DCL1-D1D2 concentrate in nuclear dicing bodies in which DCL1 and HYL1 colocalize. We show further that protein-protein interaction is mainly mediated by DCL1-D2, while DCL1-D1 plays a major role in binding of pri-miRNAs. These results suggest parallel roles between C-terminal dsRBDs of DCL1 and N-terminal dsRBDs of HYL1 and support a model in which Arabidopsis pri-miRNAs are recruited to dicing bodies through functionally divergent dsRBDs of microprocessor for accurate processing of plant pri-miRNAs. |
| 学科主题 | Plant Sciences |
| 类目[WOS] | Plant Sciences |
| 关键词[WOS] | MICRORNA BIOGENESIS ; ARABIDOPSIS-THALIANA ; PROTEIN HYL1 ; PRI-MIRNA ; PLANT ; GENE ; COMPLEX ; ROLES ; DCL1 ; PRECURSORS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000323759200010 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/311]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Liu, Q,Yan, QQ,Liu, Y,et al. Complementation of Hyponastic Leaves1 by Double-Strand RNA-Binding Domains of Dicer-Like1 in Nuclear Dicing Bodies[J]. PLANT PHYSIOLOGY,2013,163(1):108-117. |
| APA | Liu, Q.,Yan, QQ.,Liu, Y.,Hong, F.,Sun, ZF.,...&Fang, YD.(2013).Complementation of Hyponastic Leaves1 by Double-Strand RNA-Binding Domains of Dicer-Like1 in Nuclear Dicing Bodies.PLANT PHYSIOLOGY,163(1),108-117. |
| MLA | Liu, Q,et al."Complementation of Hyponastic Leaves1 by Double-Strand RNA-Binding Domains of Dicer-Like1 in Nuclear Dicing Bodies".PLANT PHYSIOLOGY 163.1(2013):108-117. |
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