piRNA-Triggered MIWI Ubiquitination and Removal by APC/C in Late Spermatogenesis
文献类型：期刊论文
| 作者 | Zhao, S; Gou, LT; Zhang, M; Zu, LD; Hua, MM; Hua, Y; Shi, HJ; Li, Y; Li, JS; Li, DS |
| 刊名 | DEVELOPMENTAL CELL
 |
| 出版日期 | 2013 |
| 卷号 | 24期号:1页码:13-25 |
| 通讯作者 | Wang, ED (reprint author), Chinese Acad Sci, Grad Univ, State Key Lab Mol Biol, Shanghai 200031, Peoples R China.,edwang@sibcb.ac.cn ; mfliu@sibcb.ac.cn |
| 英文摘要 | The PIWI/PIWI-interacting RNA (piRNA) machinery has been well documented to maintain genome integrity by silencing transposons in animal germ cells. Recent studies have advanced our understanding of the biogenesis and function of this machinery; however, its metabolism has remained largely unexplored. Here, we show that murine PIWI (MIWI) is degraded through the APC/C-26S proteasome pathway and that piRNAs play an indispensable role in this process by enhancing MIWI interaction with an APC/C substrate-binding subunit. Interestingly, piRNA-triggered MIWI destruction occurs in late spermatids, which in turn leads to piRNA elimination, suggesting a feedforward mechanism for coordinated removal of the MIWI/piRNA machinery at a specific developmental stage. Importantly, the proper removal of MIWI/piRNA is essential for sperm maturation. Together, our results reveal a role for piRNAs in regulating the clearance of the MIWI/piRNA machinery via the ubiquitin-proteosome pathway and demonstrate the critical importance of proper temporal regulation of MIWI/piRNA in male germ cell development. |
| 学科主题 | Cell Biology; Developmental Biology |
| 类目[WOS] | Cell Biology ; Developmental Biology |
| 关键词[WOS] | ANAPHASE-PROMOTING COMPLEX ; SMALL RNAS ; DEPENDENT PROTEOLYSIS ; HUMAN-CELLS ; PIWI ; DROSOPHILA ; PROTEIN ; MILI ; GERMLINE ; GENE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000316305200002 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/321]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhao, S,Gou, LT,Zhang, M,et al. piRNA-Triggered MIWI Ubiquitination and Removal by APC/C in Late Spermatogenesis[J]. DEVELOPMENTAL CELL,2013,24(1):13-25. |
| APA | Zhao, S.,Gou, LT.,Zhang, M.,Zu, LD.,Hua, MM.,...&Liu, MF.(2013).piRNA-Triggered MIWI Ubiquitination and Removal by APC/C in Late Spermatogenesis.DEVELOPMENTAL CELL,24(1),13-25. |
| MLA | Zhao, S,et al."piRNA-Triggered MIWI Ubiquitination and Removal by APC/C in Late Spermatogenesis".DEVELOPMENTAL CELL 24.1(2013):13-25. |
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