Constitutive and tumor necrosis factor-alpha-induced activation of nuclear factor-kappa B in adenomyosis and its inhibition by andrographolide
文献类型：期刊论文
| 作者 | Li, B; Chen, M; Liu, XS; Guo, SW |
| 刊名 | FERTILITY AND STERILITY
 |
| 出版日期 | 2013 |
| 卷号 | 100期号:2页码:568-577 |
| 关键词 | Adenomyosis andrographolide constitutive activation COX-2 dysmenorrhea NF-kappa B severity tissue factor VEGF |
| 通讯作者 | Guo, SW (reprint author), Fudan Univ, Shanghai Obstet & Gynecol Hosp, 419 Fangxie Rd, Shanghai 200011, Peoples R China.,hoxa10@gmail.com |
| 英文摘要 | Objective: To investigate the action of nuclear factor (NF)-kappa B in adenomyosis and evaluate the potential therapeutic effect of andrographolide on tumor necrosis factor (TNF)-alpha-induced expression of NF-kappa B-mediated genes cyclooxygease-2 (COX-2), vascular endothelial growth factor (VEGF), and tissue factor (TF) in adenomyotic stromal cells. Design: Laboratory study using human tissues. Setting: Academic hospital. Patient(s): Twenty-nine patients (cases) with histologically confirmed adenomyosis and 14 (controls) without adenomyosis or endometriosis. Intervention(s): Endometrial stromal cells derived from tissue samples harvested from both cases and controls were subjected to electrophoretic mobility shift assay, and gene and protein expression analyses. Main Outcome Measure(s): The NF-kappa B DNA-binding activity and protein levels of NF-kappa B subunits p50 and p65 and the messenger RNA (mRNA) and protein levels of NF-kappa B-mediated genes COX-2, VEGF, and TF in cases and controls, and their changes after stimulation with TNF-alpha and treatment with andrographolide. Result(s): The constitutive NF-kappa B DNA-binding activity and protein expression levels of p50 and p65, and mRNA and protein levels of COX-2, VEGF, and TF in cases were significantly higher than that of controls. The binding activity level correlated positively with dysmenorrhea severity in cases. The TNF-alpha stimulation further increased the binding activity, and the mRNA and protein levels of COX-2, VEGF, and TF, but treatment with andrographolide significantly reduced them. Conclusion(s): NF-kappa B may be a pivotal transcription factor involved in the development of adenomyosis. Targeting NF-kappa B with inhibitors, like andrographolide, may hold promises of treating adenomyosis. (C) 2013 by American Society for Reproductive Medicine. |
| 学科主题 | Obstetrics & Gynecology; Reproductive Biology |
| 类目[WOS] | Obstetrics & Gynecology ; Reproductive Biology |
| 关键词[WOS] | GROWTH/PERMEABILITY FACTOR EXPRESSION ; INTESTINAL EPITHELIAL-CELLS ; PROSTATE-CANCER CELLS ; TISSUE FACTOR ; UTERINE CONTRACTILITY ; GENE-EXPRESSION ; STROMAL CELLS ; IN-VITRO ; CYCLOOXYGENASE-2 EXPRESSION ; MATRIX METALLOPROTEINASE-2 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000322633200050 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/331]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Li, B,Chen, M,Liu, XS,et al. Constitutive and tumor necrosis factor-alpha-induced activation of nuclear factor-kappa B in adenomyosis and its inhibition by andrographolide[J]. FERTILITY AND STERILITY,2013,100(2):568-577. |
| APA | Li, B,Chen, M,Liu, XS,&Guo, SW.(2013).Constitutive and tumor necrosis factor-alpha-induced activation of nuclear factor-kappa B in adenomyosis and its inhibition by andrographolide.FERTILITY AND STERILITY,100(2),568-577. |
| MLA | Li, B,et al."Constitutive and tumor necrosis factor-alpha-induced activation of nuclear factor-kappa B in adenomyosis and its inhibition by andrographolide".FERTILITY AND STERILITY 100.2(2013):568-577. |
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