Melatonin inhibits the proliferation of human osteosarcoma cell line MG-63
文献类型：期刊论文
| 作者 | Liu, LF; Xu, Y; Reiter, RJ |
| 刊名 | BONE
 |
| 出版日期 | 2013 |
| 卷号 | 55期号:2页码:432-438 |
| 关键词 | Melatonin Osteosarcoma Proliferation MG-63 Cell cycle |
| 通讯作者 | Liu, LF (reprint author), Tongji Univ, Sch Med, East Hosp, Dept Orthopaed,Trauma Ctr, 150 Jimo Rd, Shanghai 200120, Peoples R China.,li_feng_liu@hotmail.com |
| 英文摘要 | It seems established that the onset of osteosarcoma and the reduction in melatonin production run in parallel; this suggests that the decline in the cancer-inhibiting agent, melatonin, may contribute to the occurrence of osteosarcoma and that melatonin supplementation may have promise for preventing the development and progression of this condition. There is, however, no direct evidence regarding an antiproliferative effect of melatonin in osteosarcoma cells. In the current study, we examined whether melatonin inhibits the proliferation of human osteosarcoma cell line MG-63. MTT staining showed that at 4 mM-10 mM concentrations, melatonin significantly reduced the MG-63 cell proliferation in a dose-dependent and time-dependent manner. Flow cytometry documented that 4 mM melatonin significantly increased the fraction of cells in the G(0)/G(1) phase of the cell cycle, while simultaneously reducing the proportion in the S and G(2)/M phases. Western blot and real-time PCR analyses further confirmed that melatonin's inhibitory effect was possibly because of downregulation of cyclin D1 and CDK4, related to the G(1) phase, and of cyclin B1 and CDK1, related to the G(2)/M phase. There was no downregulation of cyclin E, CDK2, and cyclin A, which are related to G(1)/S transition and S phase. These findings provide evidence that melatonin may significantly inhibit human osteosarcoma cell proliferation in a dose-dependent and time-dependent manner and this inhibition involves the downregulation of cyclin D1, CDK4, cyclin B1 and CDK1. Crown Copyright (C) 2013 Published by Elsevier Inc. All rights reserved. |
| 学科主题 | Endocrinology & Metabolism |
| 类目[WOS] | Endocrinology & Metabolism |
| 关键词[WOS] | BREAST-CANCER CELLS ; GROWTH-INHIBITION ; CYCLIN-A ; MAMMALIAN FIBROBLASTS ; DOWN-REGULATION ; BONE-MARROW ; RECEPTORS ; APOPTOSIS ; PATHWAYS ; ARREST |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000320896600024 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/332]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Liu, LF,Xu, Y,Reiter, RJ. Melatonin inhibits the proliferation of human osteosarcoma cell line MG-63[J]. BONE,2013,55(2):432-438. |
| APA | Liu, LF,Xu, Y,&Reiter, RJ.(2013).Melatonin inhibits the proliferation of human osteosarcoma cell line MG-63.BONE,55(2),432-438. |
| MLA | Liu, LF,et al."Melatonin inhibits the proliferation of human osteosarcoma cell line MG-63".BONE 55.2(2013):432-438. |
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