Efficiencies of Growth and Angiostatin Expression in Cultures of Pichia pastoris Fed with Mixed Carbon Sources
文献类型：期刊论文
| 作者 | Xie, J; Yang, R; Zhou, Q; Du, P; Gan, R; Ye, Q |
| 刊名 | CHEMICAL AND BIOCHEMICAL ENGINEERING QUARTERLY
 |
| 出版日期 | 2013 |
| 卷号 | 27期号:2页码:235-244 |
| 关键词 | Mass balance Pichia pastoris glycerol lactate angiostatin |
| 通讯作者 | Ye, Q (reprint author), E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China.,qye@ecust.edu.cn |
| 英文摘要 | Different carbon sources, glycerol, sorbitol, acetate and lactate were added together with methanol in the induction phase of Pichia pastoris culture for angiostatin production in a 5-L fermentor. Mass balances were carried out for different processes, yield coefficients were discussed and stoichiometric coefficients were estimated to compare the efficiency of different carbon sources. Glycerol had the highest reduction degree among the four non-methanol carbon sources, as well as higher energetic yield coefficient for biomass production (eta) and lower respiratory quotient (RQ), indicating that glycerol was a more efficient carbon source for biomass formation than the other three non-methanol carbon sources. Lactic acid, a non-repression carbon sources, had lower reduction degree, but achieved the highest energetic yield coefficient for angiostatin production (xi(P)). |
| 学科主题 | Biotechnology & Applied Microbiology; Engineering |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Engineering, Chemical |
| 关键词[WOS] | RECOMBINANT PROTEIN EXPRESSION ; YEAST ; FERMENTATION ; METHANOL ; GENE ; CULTIVATION ; STRATEGIES ; SECRETION ; BALANCE ; MODEL |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000322055200013 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/333]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Xie, J,Yang, R,Zhou, Q,et al. Efficiencies of Growth and Angiostatin Expression in Cultures of Pichia pastoris Fed with Mixed Carbon Sources[J]. CHEMICAL AND BIOCHEMICAL ENGINEERING QUARTERLY,2013,27(2):235-244. |
| APA | Xie, J,Yang, R,Zhou, Q,Du, P,Gan, R,&Ye, Q.(2013).Efficiencies of Growth and Angiostatin Expression in Cultures of Pichia pastoris Fed with Mixed Carbon Sources.CHEMICAL AND BIOCHEMICAL ENGINEERING QUARTERLY,27(2),235-244. |
| MLA | Xie, J,et al."Efficiencies of Growth and Angiostatin Expression in Cultures of Pichia pastoris Fed with Mixed Carbon Sources".CHEMICAL AND BIOCHEMICAL ENGINEERING QUARTERLY 27.2(2013):235-244. |
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