Differentiation of Reprogrammed Mouse Cardiac Fibroblasts into Functional Cardiomyocytes
文献类型：期刊论文
| 作者 | Jiang, B; Dong, HY; Li, QP; Yu, Y; Zhang, ZF; Zhang, YZ; Wang, G; Zhang, ZM |
| 刊名 | CELL BIOCHEMISTRY AND BIOPHYSICS
 |
| 出版日期 | 2013 |
| 卷号 | 66期号:2页码:309-318 |
| 关键词 | Cardiac fibroblasts Induced pluripotent stem cells Directed differentiation Cardiomyocyte Myocardial regeneration |
| 通讯作者 | Zhang, ZM (reprint author), Nanjing Med Univ, Dept Thorac & Cardiovasc Surg, Affiliated Hosp 1, Nanjing 210029, Jiangsu, Peoples R China.,gwang22@sibs.ac.cn ; zhang_zhongming@yahoo.com.cn |
| 英文摘要 | Fibroblasts can be reprogrammed by ectopic expression of reprogramming factors to yield induced pluripotent stem (iPS) cells that are capable of transdifferentiating into diverse types of somatic cell lines. In this study, we examined if functional cardiomyocytes (CMs) can be produced from mouse cardiac fibroblasts (CFs), using iPS cell factor-based reprogramming. CFs were isolated from Oct4-GFP-C57 mice and infected with a retrovirus expressing the Yamanaka reprogramming factors, Oct4, Sox2, Klf4, and c-Myc to reprogram the CFs into a CF-iPS cell line. Primary mouse embryonic fibroblast cells (MEFs) were used as a control. We found that the dedifferentiated CF-iPS cells showed similar biological characteristics (morphology, pluripotent factor expression, and methylation level) as embryonic stem cells (ESs) and MEF-iPS cells. We used the classical embryoid bodies (EBs)-based method and a transwell CM co-culture system to simulate the myocardial paracrine microenvironment for performing CF-iPS cell cardiogenic differentiation. Under this simulated myocardial microenvironment, CF-iPS cells formed spontaneously beating EBs. The transdifferentiated self-beating cells expressed cardiac-specific transcription and structural factors and also displayed typical myocardial morphology and electrophysiological characteristics. CFs can be dedifferentiated into iPS cells and further transdifferentiated into CMs. CFs hold great promise for CM regeneration as an autologous cell source for functional CM in situ without the need for exogenous cell transplantation in ischemic heart disease. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics; Cell Biology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics ; Cell Biology |
| 关键词[WOS] | PLURIPOTENT STEM-CELLS ; SOMATIC-CELLS ; HEART ; GENERATION ; INFARCTION ; DISEASE ; ORIGIN ; MURINE ; REPAIR ; TISSUE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000319082300009 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/334]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Jiang, B,Dong, HY,Li, QP,et al. Differentiation of Reprogrammed Mouse Cardiac Fibroblasts into Functional Cardiomyocytes[J]. CELL BIOCHEMISTRY AND BIOPHYSICS,2013,66(2):309-318. |
| APA | Jiang, B.,Dong, HY.,Li, QP.,Yu, Y.,Zhang, ZF.,...&Zhang, ZM.(2013).Differentiation of Reprogrammed Mouse Cardiac Fibroblasts into Functional Cardiomyocytes.CELL BIOCHEMISTRY AND BIOPHYSICS,66(2),309-318. |
| MLA | Jiang, B,et al."Differentiation of Reprogrammed Mouse Cardiac Fibroblasts into Functional Cardiomyocytes".CELL BIOCHEMISTRY AND BIOPHYSICS 66.2(2013):309-318. |
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