GABA exists as a negative regulator of cell proliferation in spermaogonial stem cells
文献类型：期刊论文
| 作者 | Du, Y; Du, Z; Zheng, HP; Wang, D; Li, SF; Yan, YC; Li, YP |
| 刊名 | CELLULAR & MOLECULAR BIOLOGY LETTERS
 |
| 出版日期 | 2013 |
| 卷号 | 18期号:2页码:149-162 |
| 关键词 | GABA GABAA receptor Spermatogonial stem cells Proliferation Muscimol Testes |
| 通讯作者 | Li, YP (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Key Lab Mol Androl, State Key Lab Cell Biol,Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,yipingli@sibs.ac.cn |
| 英文摘要 | gamma-amino butyric acid (GABA) is the main inhibitory neurotransmitter in the mammalian central nervous system. GABA is also found in many peripheral tissues, where it has important functions during development. Here, we identified the existence of the GABA system in spermatogonial stem cells (SSCs) and found that GABA negatively regulates SSC proliferation. First, we demonstrated that GABA and its synthesizing enzymes were abundant in the testes 6 days postpartum (dpp), suggesting that GABA signaling regulates SSCs function in vivo. In order to directly examine the effect of GABA on SSC proliferation, we then established an in vitro culture system for long-term expansion of SSCs. We showed that GABA(A) receptor subunits, including alpha 1, alpha 5, beta 1, beta 2, beta 3 and gamma 3, the synthesizing enzyme GAD67, and the transporter GAT-1, are expressed in SSCs. Using phosphorylated histone H3 (pH3) staining, we demonstrated that GABA or the GABA(A)R-specific agonist muscimol reduced the proliferation of SSCs. This GABA regulation of SSC proliferation was shown to be independent of apoptosis using the TUNEL assay. These results suggest that GABA acts as a negative regulator of SSC proliferation to maintain the homeostasis of spermatogenesis in the testes. |
| 学科主题 | Biochemistry & Molecular Biology; Cell Biology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Cell Biology |
| 关键词[WOS] | SELF-RENEWAL ; NEUROTROPHIC FACTOR ; ACROSOME REACTION ; MOUSE TESTIS ; IN-VITRO ; EXPRESSION ; RAT ; RECEPTORS ; GENES ; LINE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000318869200001 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/335]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Du, Y,Du, Z,Zheng, HP,et al. GABA exists as a negative regulator of cell proliferation in spermaogonial stem cells[J]. CELLULAR & MOLECULAR BIOLOGY LETTERS,2013,18(2):149-162. |
| APA | Du, Y.,Du, Z.,Zheng, HP.,Wang, D.,Li, SF.,...&Li, YP.(2013).GABA exists as a negative regulator of cell proliferation in spermaogonial stem cells.CELLULAR & MOLECULAR BIOLOGY LETTERS,18(2),149-162. |
| MLA | Du, Y,et al."GABA exists as a negative regulator of cell proliferation in spermaogonial stem cells".CELLULAR & MOLECULAR BIOLOGY LETTERS 18.2(2013):149-162. |
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