IPP5 inhibits neurite growth in primary sensory neurons by maintaining TGF-beta/Smad signaling
文献类型：期刊论文
| 作者 | Han, QJ; Gao, NN; Ma, GQ; Zhang, ZN; Yu, WH; Pan, J; Wang, Q; Zhang, X; Bao, L |
| 刊名 | JOURNAL OF CELL SCIENCE
 |
| 出版日期 | 2013 |
| 卷号 | 126期号:2页码:542-553 |
| 关键词 | IPP5 Neurite growth PP1 TGF-beta/Smad signaling DRG neuron |
| 通讯作者 | Bao, L (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Cell Biol, Shanghai 200031, Peoples R China.,baolan@sibcb.ac.cn |
| 英文摘要 | During nerve regeneration, neurite growth is regulated by both intrinsic molecules and extracellular factors. Here, we found that inhibitor 5 of protein phosphatase 1 (IPP5), a newly identified inhibitory subunit of protein phosphatase 1 (PP1), inhibited neurite growth in primary sensory neurons as an intrinsic regulator. IPP5 was highly expressed in the primary sensory neurons of rat dorsal root ganglion (DRG) and was downregulated after sciatic nerve axotomy. Knocking down IPP5 with specific shRNA increased the length of the longest neurite, the total neurite length and the number of neurite ends in cultured rat DRG neurons. Mutation of the PP1-docking motif K(8)IQF(11) or the PP1-inhibiting motif at Thr(34) eliminated the IPP5-induced inhibition of neurite growth. Furthermore, biochemical experiments showed that IPP5 interacted with type I transforming growth factor-beta receptor (T beta RI) and PP1 and enhanced transforming growth factor-beta (TGF-beta)/Smad signaling in a PP1-dependent manner. Overexpressing IPP5 in DRG neurons aggravated TGF-beta-induced inhibition of neurite growth, which was abolished by blocking PP1 or IPP5 binding to PP1. Blockage of TGF-beta signaling with the TbRI inhibitor SB431542 or Smad2 shRNA attenuated the IPP5-induced inhibition of neurite growth. Thus, these data indicate that selectively expressed IPP5 inhibits neurite growth by maintaining TGF-beta signaling in primary sensory neurons. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | AXON REGENERATION ; PROTEIN PHOSPHATASE-1 ; GENE-EXPRESSION ; CELL-DIFFERENTIATION ; IN-VIVO ; PHOSPHORYLATION ; PATHWAY ; CNS ; DEPHOSPHORYLATION ; MORPHOGENESIS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000316945600017 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/346]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Han, QJ,Gao, NN,Ma, GQ,et al. IPP5 inhibits neurite growth in primary sensory neurons by maintaining TGF-beta/Smad signaling[J]. JOURNAL OF CELL SCIENCE,2013,126(2):542-553. |
| APA | Han, QJ.,Gao, NN.,Ma, GQ.,Zhang, ZN.,Yu, WH.,...&Bao, L.(2013).IPP5 inhibits neurite growth in primary sensory neurons by maintaining TGF-beta/Smad signaling.JOURNAL OF CELL SCIENCE,126(2),542-553. |
| MLA | Han, QJ,et al."IPP5 inhibits neurite growth in primary sensory neurons by maintaining TGF-beta/Smad signaling".JOURNAL OF CELL SCIENCE 126.2(2013):542-553. |
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